Nedocromil Sodium Inhibits Canine Adenovirus Bronchiolitis in Beagle Puppies

Autor: M. C. Bowers, R. C. Lantz, Huizhong Chen, Richard J. Lemen, D. E. Bice, Natalie Weger, Mark L. Witten, B. A. Muggenburg, K. A. Anderson
Rok vydání: 2000
Předmět:
Nedocromil
medicine.medical_specialty
Pathology
Neutrophils
040301 veterinary sciences
Adenoviridae Infections
medicine.medical_treatment
Cell Count
Respiratory Mucosa
Adenoviruses
Canine
Toxicology
030226 pharmacology & pharmacy
Beagle
Gastroenterology
Pathology and Forensic Medicine
0403 veterinary science
03 medical and health sciences
Dogs
0302 clinical medicine
Internal medicine
medicine
Animals
Bronchiolitis
Viral
Anti-Asthmatic Agents
Nedocromil Sodium
Lung
Molecular Biology
Saline
medicine.diagnostic_test
business.industry
Respiratory disease
04 agricultural and veterinary sciences
Cell Biology
respiratory system
medicine.disease
Neutrophilia
Respiratory Function Tests
respiratory tract diseases
Disease Models
Animal
Bronchoalveolar lavage
Bronchiolitis
medicine.symptom
business
Bronchoalveolar Lavage Fluid
medicine.drug
Zdroj: Toxicologic Pathology. 28:317-325
ISSN: 1533-1601
0192-6233
DOI: 10.1177/019262330002800212
Popis: Nedocromil sodium is a nonsteroidal anti-inflammatory drug used to control asthmatic attacks. Our hypothesis is that nedocromil sodium inhibits virus-induced airway inflammation, a common trigger of asthma. We nebulized nedocromil sodium into beagle dogs (n = 10, mean +/- SEM ages: 149 +/- 13 days) before and after inoculation with canine adenovirus type 2 (CAV2). Control dogs (n = 10) received saline aerosols and were either infected with CAV2 (Sal/CAV2, n = 7, mean +/- SEM ages: 140 +/- 11 days) or were not infected (Sal/Sal, n = 3, ages: 143 +/- 0 days). All dogs were anesthetized with choralose (80 mg/kg i.v.), intubated, and mechanically ventilated. Pulmonary function tests and bronchoalveolar lavage (BAL) were performed using standard techniques. Pulmonary function tests revealed no significant change between the nedocromil sodium and non-nedocromil-treated groups. The percentage of infected bronchioles was quantitated as the number of inflamed airways of 40 bronchioles examined times 100 for each dog. Nedocromil-treated dogs had significantly (p < 0.05) less mucosal inflammation (mean +/- SEM, 39% +/- 5%), epithelial denudation (36% +/- 5%), and BAL neutrophilia (11 +/- 3) than did Sal/CAV2 dogs (51% +/- 6%, 57% +/- 4%, and 33% +/- 8%, respectively). We concluded that pretreatment with nedocromil sodium aerosols attenuated CAV2-induced airway inflammation in these beagle puppies.
Databáze: OpenAIRE
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