Valorization of Biomasses from Energy Crops for the Discovery of Novel Thermophilic Glycoside Hydrolases through Metagenomic Analysis
| Autor: | Roberta Iacono, Andrea Strazzulli, Rosa Giglio, Federica Bitetti, Beatrice Cobucci-Ponzano, Marco Moracci |
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| Přispěvatelé: | Iacono, Roberta, Strazzulli, Andrea, Giglio, Rosa, Bitetti, Federica, Cobucci-Ponzano, Beatrice, Moracci, Marco |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Crops
Agricultural Glycoside Hydrolases Organic Chemistry Archaea Astrobiology Extremophiles Hyperthermophiles Starch alpha-Glucosidases General Medicine Archaea Catalysis CAZymes extremozymes archaea enrichments Computer Science Applications Inorganic Chemistry Biomass Metagenomics Physical and Theoretical Chemistry Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 18; Pages: 10505 |
| Popis: | The increasing interest for environmentally friendly technologies is driving the transition from fossil-based economy to bioeconomy. A key enabler for circular bioeconomy is to valorize renewable biomasses as feedstock to extract high value-added chemicals. Within this transition the discovery and the use of robust biocatalysts to replace toxic chemical catalysts play a significant role as technology drivers. To meet both the demands, we performed microbial enrichments on two energy crops, used as low-cost feed for extremophilic consortia. A culture-dependent approach coupled to metagenomic analysis led to the discovery of more than 300 glycoside hydrolases and to characterize a new α-glucosidase from an unknown hyperthermophilic archaeon. Aglu1 demonstrated to be the most active archaeal GH31 on 4Np-α-Glc and it showed unexpected specificity vs. kojibiose, revealing to be a promising candidate for biotechnological applications such as the liquefaction/saccharification of starch. |
| Databáze: | OpenAIRE |
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