Molecular typing and diagnosis of Anaplasma spp. closely related to Anaplasma phagocytophilum in ruminants from Tunisia
| Autor: | Rosanna Zobba, Narjesse El Mabrouk, Alberto Alberti, Ali Bouattour, Lilia Messadi, Mariem Saidani, Hanène Belkahia, Mariem Ben Hassen, Skander Bouattour, Mourad Ben Said |
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| Přispěvatelé: | École Nationale de Médecine Vétérinaire de Sidi Thabet, Ministère de l’Agriculture, des Ressources Hydrauliques et de la Pêche Maritime [Tunisie], University of Sassari, Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP), This study was supported by the research project 'PS1.3.023–RESTUS' funded by the European Neighbourhood and Partnership Instrument (ENPI) − Transboundary Cooperation (TC) − Italy-Tunisia2007-2013, the 'Laboratoire d’épidémiologie d’infections enzootiques des herbivores en Tunisie' (LR02AGR03), funded by the Ministry of Higher Education and Scientific Research of Tunisia, and the research project 'Epidémiologie de maladies bactériennes à transmission vectorielle des herbivores' (06-680-0029) funded by the Ministry of Agriculture of Tunisia., The authors would like to thank Dr. Leïla Sayeh, Dr. Saber Hdhiri, Dr. Aymen Sahbani, Dr. Tarak Blaïech, Dr. Oussama Mathlouthi, Dr. Said Jaajaa and Dr. Taoufik Ben Hamida and their technicians for their help and facilitating the access to the farmers. |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
MESH: Ehrlichiosis/epidemiology Veterinary medicine Anaplasmosis MESH: Sequence Analysis DNA MESH: Coinfection/veterinary animal diseases Prevalence MESH: Tunisia/epidemiology Polymerase Chain Reaction MESH: Anaplasma phagocytophilum/genetics RNA Ribosomal 16S Co-occurrence MESH: Coinfection/diagnosis MESH: Ehrlichiosis/veterinary MESH: Animals MESH: Genetic Variation 16S rRNA PCR-RFLP MESH: Phylogeny Phylogeny MESH: Coinfection/epidemiology 2. Zero hunger Genetics Coinfection Goats Ruminants 3. Good health MESH: RNA Ribosomal 16S MESH: Cattle Infectious Diseases [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology MESH: Anaplasma phagocytophilum/classification MESH: Coinfection/microbiology MESH: Ehrlichiosis/diagnosis Restriction fragment length polymorphism MESH: Goat Diseases/epidemiology MESH: Sheep Diseases/microbiology Anaplasma phagocytophilum DNA Bacterial MESH: Goat Diseases/diagnosis Anaplasma Tunisia 030106 microbiology Sheep Diseases MESH: Sheep MESH: Anaplasmosis/epidemiology MESH: Ehrlichiosis/microbiology MESH: Anaplasmosis/diagnosis Biology Microbiology 03 medical and health sciences Phylogenetics MESH: Sheep Diseases/epidemiology parasitic diseases MESH: Goat Diseases/microbiology medicine Animals MESH: Anaplasma/isolation & purification Anaplasma phagocytophilum-like 1 and Anaplasma phagocytophilum-like 2 MESH: Anaplasma/genetics Goat Diseases Sheep [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health MESH: Molecular Typing fungi Ehrlichiosis Genetic Variation Sequence Analysis DNA MESH: Polymerase Chain Reaction/veterinary biology.organism_classification medicine.disease bacterial infections and mycoses MESH: DNA Bacterial MESH: Goats/microbiology Molecular Typing MESH: Anaplasma/classification Insect Science MESH: Anaplasmosis/microbiology bacteria Parasitology Cattle Nested polymerase chain reaction Molecular discrimination MESH: Anaplasma phagocytophilum/isolation & purification MESH: Sheep Diseases/diagnosis |
| Zdroj: | Ticks and Tick-borne Diseases Ticks and Tick-borne Diseases, Elsevier, 2017, 8 (3), pp.412-422. ⟨10.1016/j.ttbdis.2017.01.005⟩ |
| ISSN: | 1877-959X |
| Popis: | International audience; Accurate diagnosis of animal and zoonotic diseases, such as granulocytic anaplasmosis, is crucial to estimate risk during control programs. In this study, 16S rRNA nested PCR and RFLP assay were combined to investigate the presence of Anaplasma phagocytophilum and genetically related strains (namely A. phagocytophilum-like 1 and 2) in 936 Tunisian ruminants. By using this method, A. phagocytophilum was not detected in any of the tested animals, while A. phagocytophilum-like 1 and A. phagocytophilum-like 2 were detected at variable prevalence rates in sheep, goats and cattle at coinfection rates respectively of 3.9, 2.5 and 0.5%. Sequence analysis validated RFLP data, and confirmed the co-occurrence of two potentially novel species closely related to A. phagocytophilum in Tunisian ruminants. Phylogeny indicated the presence of genetic variants shared by different ruminant species for each type of A. phagocytophilum-like strains. Results raise concern on the use and interpretation of indirect and direct tests traditionally employed for detecting pathogenic A. phagocytophilum strains in ruminants and in other vertebrates' species, and provide additional background to improve classification of bacterial species closely related to A. phagocytophilum, and to reconstruct their evolutionary history. (C) 2017 Elsevier GmbH. All rights reserved. |
| Databáze: | OpenAIRE |
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