The cytotoxicity of eosinophil cationic protein/ribonuclease 3 on eukaryotic cell lines takes place through its aggregation on the cell membrane
| Autor: | Marcel Jiménez, Claudi M. Cuchillo, Ester Boix, Jordi Aleu, M.V. Nogués, Susanna Navarro |
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| Rok vydání: | 2007 |
| Předmět: |
Programmed cell death
Cell Membrane Permeability Time Factors RNase P media_common.quotation_subject Lipid Bilayers education Cell Intracellular Space HL-60 Cells Phosphatidylserines Protein aggregation Cell Line Cell membrane Cellular and Molecular Neuroscience fluids and secretions medicine Humans Ribonuclease Protein Structure Quaternary Internalization Molecular Biology media_common Pharmacology Eosinophil cationic protein Microscopy Confocal Cell Death biology Cell Cycle Cell Membrane Eosinophil Cationic Protein Cell Biology Caspase Inhibitors Cell biology Protein Transport Eukaryotic Cells medicine.anatomical_structure biology.protein RNA Molecular Medicine Calcium Reactive Oxygen Species HeLa Cells |
| Zdroj: | Cellular and Molecular Life Sciences. 65:324-337 |
| ISSN: | 1420-9071 1420-682X |
| DOI: | 10.1007/s00018-007-7499-7 |
| Popis: | Human eosinophil cationic protein (ECP)/ ribonuclease 3 (RNase 3) is a protein secreted from the secondary granules of activated eosinophils. Specific properties of ECP contribute to its cytotoxic activities associated with defense mechanisms. In this work the ECP cytotoxic activity on eukaryotic cell lines is analyzed. The ECP effects begin with its binding and aggregation to the cell surface, altering the cell membrane permeability and modifying the cell ionic equilibrium. No internalization of the protein is observed. These signals induce cell-specific morphological and biochemical changes such as chromatin condensation, reversion of membrane asymmetry, reactive oxygen species production and activation of caspase-3-like activity and, eventually, cell death. However, the ribonuclease activity component of ECP is not involved in this process as no RNA degradation is observed. In summary, the cytotoxic effect of ECP is attained through a mechanism different from that of other cytotoxic RNases and may be related with the ECP accumulation associated with the inflammatory processes, in which eosinophils are present. |
| Databáze: | OpenAIRE |
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