Genetic variants in protein kinase C zeta gene and type 2 diabetes risk: a case-control study of a Chinese Han population
| Autor: | Ling Zhou, Jin-luo Cheng, Lei Qin, Jihong Hu, Yunfeng Du, Xue-ji Wu, Jin-yi Zhou, Mo-yu Shen, Jingsong Wang |
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| Rok vydání: | 2008 |
| Předmět: |
China
medicine.medical_specialty Genotype Endocrinology Diabetes and Metabolism Population Single-nucleotide polymorphism Type 2 diabetes Biology Polymerase Chain Reaction Polymorphism Single Nucleotide Interviews as Topic Endocrinology Asian People Risk Factors Polymorphism (computer science) Surveys and Questionnaires Diabetes mellitus Internal medicine Internal Medicine medicine Humans Genetic Predisposition to Disease International HapMap Project education Protein Kinase C DNA Primers Genetics education.field_of_study Haplotype Genetic Variation Type 2 Diabetes Mellitus medicine.disease Diabetes Mellitus Type 2 Case-Control Studies Polymorphism Restriction Fragment Length |
| Zdroj: | Diabetes/Metabolism Research and Reviews. 24:480-485 |
| ISSN: | 1520-7560 1520-7552 |
| Popis: | Background Previous investigations of the protein kinase C zeta (PRKCZ) gene, a susceptibility factor for type 2 diabetes mellitus (T2DM), have focussed on its single nucleotide polymorphisms (SNPs). In a departure from these earlier studies, we tested six tagging markers of PRKCZ based on the Hapmap database to evaluate their association with T2DM. Methods PCR-RFLP was performed to test the genotypes in type 2 diabetes patients (n = 343) and normal controls (n = 429). Insulin-related indexes were calculated from levels of fasting plasma insulin (FPI) and fasting plasma glucose (FPG). Results The variant genotypes, rs3753242TT, rs2503706CT, rs2503706CT/ TT and rs262654TT were associated with T2DM. And the significance remained for rs3753242 TT (p < 0.001) after the false discovery rate (FDR) correction. There was a doubling in the prevalence of the TTGCCC haplotype in the case subjects (14.2%) compared with the control subjects (6.9%) [OR (95% CI) = 2.19(1.49–3.22)]. In the control group, none of the haplotypes studied was associated with insulin-related indexes. Conclusions The PRKCZ gene variants associated with the development of T2DM in this study must be investigated in a larger population to reveal any potential effects on metabolism. Copyright © 2008 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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