The antiproliferative effect of vitamin D analogs on MCF-7 human breast cancer cells
| Autor: | Richard V. Brenner, Mohsen Shabahang, Russell J. Nauta, Robert R. Buras, Lisa M. Schumaker, Stephen R.T. Evans, Milan R. Uskokovic |
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| Rok vydání: | 1995 |
| Předmět: |
Cancer Research
medicine.medical_specialty Breast Neoplasms Adenocarcinoma Biology Binding Competitive Calcitriol receptor chemistry.chemical_compound Breast cancer Calcitriol Internal medicine Tumor Cells Cultured medicine Vitamin D and neurology Humans Biological activity medicine.disease In vitro Endocrinology Oncology chemistry MCF-7 Cancer cell Cancer research Receptors Calcitriol Growth inhibition Cell Division |
| Zdroj: | Cancer Letters. 92:77-82 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/0304-3835(95)03765-o |
| Popis: | We analyzed the antiproliferative effect of 1,25-dihydroxyvitamin D3 and four vitamin D analogs on MCF-7, a human breast cancer cell line known to express the vitamin D receptor. Growth curve studies and [3H]thymidine incorporation assays were used to assess the antiproliferative effect of 1,25-dihydroxyvitamin D3 (vitamin D), Ro 23-7553, Ro 24-5531, Ro 25-5317, and Ro 24-5583. Growth of MCF-7 cells was significantly inhibited by 1,25-dihydroxyvitamin D3 and all four analogs at 10−8 M (P < 0.05). MCF-7 cells treated with analog had significantly less [3H]thymidine incorporation than cells treated with 1,25-dihydroxyvitamin D3 (P < 0.05). The affinity of the analogs for the vitamin D receptor was similar to that of 1,25-dihydroxyvitamin D3. These results demonstrate that analogs of 1,25-dihydroxyvitamin D3 are potent antiproliferative agents on human breast cancer cells and that this activity is likely mediated through the vitamin D receptor. |
| Databáze: | OpenAIRE |
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