Detection and molecular characterization of double-stranded RNA viruses in Philippine Trichomonas vaginalis isolates
| Autor: | Mary Ann Cielo V. Relucio-San Diego, Windell L. Rivera, Christine Aubrey C. Justo, Lorenz M. Loyola |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Microbiology (medical) food.ingredient Genes Viral viruses Philippines lcsh:QR1-502 Genome Viral medicine.disease_cause Virus lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound food RNA polymerase Immunology and Microbiology(all) medicine Trichomonas vaginalis Female sex worker (FSW) Humans RNA Viruses Immunology and Allergy Gene Phylogeny RNA Double-Stranded Totiviridae Sex Workers General Immunology and Microbiology biology Sequence Analysis RNA RNA General Medicine biology.organism_classification Virology 030104 developmental biology Infectious Diseases chemistry Trichomonas vaginalis virus/Trichomonasvirus Double-stranded RNA viruses Capsid Proteins Female Trichomonasvirus |
| Zdroj: | Journal of Microbiology, Immunology and Infection, Vol 50, Iss 5, Pp 669-676 (2017) |
| ISSN: | 1684-1182 |
| DOI: | 10.1016/j.jmii.2015.07.016 |
| Popis: | Background/Purpose The flagellated protozoon Trichomonas vaginalis that parasitizes the urogenital tract of humans was reported to harbor double-stranded RNA (dsRNA) viruses. These viruses, identified as Trichomonas vaginalis virus (TVV), belong to the genus Trichomonasvirus of the family Totiviridae . Four species, formally recognized by the International Committee on Taxonomy of Viruses (ICTV), have been reported and distinguished by pairwise comparisons of the sequences of genes coding for major capsid protein (CP) and RNA-dependent RNA polymerase (RdRp). Methods Reverse transcription polymerase chain reaction (RT-PCR) was used to amplify the complimentary DNA of target virus genes coding for CP and RdRp. Sequence analyses confirmed the identity of the TVV isolates from T. vaginalis cultures. Results A total of 35 dsRNA viruses were identified from 18 (19%) T. vaginalis isolates. Multiple TVV species were observed in six of the 18 T. vaginalis cultures. Phylogenetic analyses show monophyly in TVV1 and TVV2 whereas TVV3 and TVV4 appear paraphyletic. The phylogeny of Philippine Trichomonasvirus reflects the global distribution of its host. Conclusion This is the first study in the Philippines and one of the two reports worldwide to detect the four TVVs and their concurrent infection in a single T. vaginalis isolate. |
| Databáze: | OpenAIRE |
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