Glomerular basement membrane injuries in IgA nephropathy evaluated by double immunostaining for α5(IV) and α2(IV) chains of type IV collagen and low-vacuum scanning electron microscopy
| Autor: | Nobuaki Yamanaka, Akira Shimizu, Kiyotaka Nagahama, T. Arai, Naomi Kuwahara, Kyoko Wakamatsu, Yukinari Masuda, Arimi Ishikawa, Kaori Ichikawa, M. Kataoka |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Collagen Type IV Male medicine.medical_specialty Pathology Adolescent Physiology Kidney Glomerulus Hydrostatic pressure urologic and male genital diseases Nephropathy Young Adult Type IV collagen Microscopy Electron Transmission Physiology (medical) Internal medicine Glomerular Basement Membrane medicine Humans Ultrasonography medicine.diagnostic_test urogenital system business.industry Glomerular basement membrane Glomerulonephritis IGA Glomerulonephritis Anatomy Middle Aged medicine.disease female genital diseases and pregnancy complications Capillaries medicine.anatomical_structure Nephrology Reticular connective tissue Microscopy Electron Scanning Glomerular Filtration Barrier Female Renal biopsy business |
| Zdroj: | Clinical and Experimental Nephrology. 19:427-435 |
| ISSN: | 1437-7799 1342-1751 |
| DOI: | 10.1007/s10157-014-1008-8 |
| Popis: | The glomerulus contains well-developed capillaries, which are at risk of injury due to high hydrostatic pressure, hyperfiltration, hypertension and inflammation. However, the pathological alterations of the injured glomerular basement membrane (GBM), the main component of the glomerular filtration barrier, are still uncertain in cases of glomerulonephritis. We examined the alterations of the GBM in 50 renal biopsy cases with IgA nephropathy (31.8 ± 17.6 years old) using double immunostaining for the α2(IV) and α5(IV) chains of type IV collagen, and examining the ultrastructural alterations by transmission electron microscopy (TEM) and low-vacuum scanning electron microscopy (LV-SEM). The GBM of IgA nephropathy cases showed various morphological and qualitative alterations. In the TEM findings, thinning, gaps, rupture, thickening with a lamellar and reticular structure and double contours were detected in the GBM. Double immunostaining for α5(IV) and α2(IV) showed thickening of the GBM with reduced α5(IV) and increased α2(IV), or mosaic images of α5(IV) and α2(IV), and holes, fractures, spiny projections and rupture of α5(IV) in the GBM. In addition, LV-SEM showed an etched image and multiple holes in a widening and wavy GBM. These findings might be associated with the development of a brittle GBM in IgA nephropathy. Glomerular basement membrane alterations were frequently noted in IgA nephropathy, and were easily evaluated by double immunostaining for α2(IV) and α5(IV) of type IV collagen and LV-SEM. The application of these analyses to human renal biopsy specimens may enhance our understanding of the alterations of the GBM that occur in human glomerular diseases. |
| Databáze: | OpenAIRE |
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