First detection of Leishmania infantum kinetoplast DNA in hair of wild mammals: Application of qPCR method to determine potential parasite reservoirs
| Autor: | J.C. Parejo, Rubén Muñoz-Madrid, Virginia Iniesta, Isabel Monroy, Victora Baz, Luis Carlos Gómez-Nieto, F. J. Serrano, Javier Fernández-Cotrina, Adela Gómez-Luque, Silvia Belinchón-Lorenzo |
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| Rok vydání: | 2013 |
| Předmět: |
Vulpes
Veterinary (miscellaneous) Leg hair Animals Wild Biology Real-Time Polymerase Chain Reaction Microbiology parasitic diseases Botany medicine Animals Parasite hosting Leishmania infantum Disease Reservoirs Mammals integumentary system Erinaceus DNA Kinetoplast DNA Protozoan biology.organism_classification Leishmania Infectious Diseases medicine.anatomical_structure Canis Spain Insect Science Kinetoplast Leishmaniasis Visceral Parasitology Hair |
| Zdroj: | Acta Tropica. 128:706-709 |
| ISSN: | 0001-706X |
| DOI: | 10.1016/j.actatropica.2013.08.009 |
| Popis: | The data presented in this paper describe the application of a method for a reliable and non-invasive diagnosis of leishmaniosis in wild reservoirs, based on the detection of Leishmania infantum kinetoplast DNA (kDNA) in hair samples by Real Time PCR (qPCR). The study has been performed on 68 ear/leg hair samples from 5 different wild species (Vulpes vulpes, Canis lupus, Martes foina, Rattus norvegicus and Erinaceus europaeus) from several geographic areas of West and North Spain. The presence of Leishmania kDNA was detected in 14 of the 68 analyzed samples, being the highest quantity of DNA observed in foxes. This is the first report of the presence of Leishmania in a hedgehog. The kDNA remained stable under the exposure of hair to different environmental conditions (freezing or high temperature, ultraviolet rays or treatment with tanning salts). This detection method could constitute a suitable alternative for the search of the parasite in wild hosts, due to the numerous advantages that hair samples present for collection, transport and storage processes. |
| Databáze: | OpenAIRE |
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