Development of an anti-Salmonella typhi ViELISA: assessment of immunocompetence in healthy donors
| Autor: | John R Jones, E. Bateman, Siraj A. Misbah, Z. Sherrell, N. Groome, C. Banner, Helen Chapel, Berne Ferry, P. Stephens, H. Lythgoe |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Salmonella Salmonella Vaccines Immunology Enzyme-Linked Immunosorbent Assay medicine.disease_cause Salmonella typhi Sensitivity and Specificity Statistics Nonparametric Microbiology Antigen Clinical Studies medicine Humans Immunology and Allergy Vi capsular polysaccharide vaccine biology business.industry Vaccination Reproducibility of Results Virology Treatment Outcome Immunization Child Preschool Immunoglobulin G Salmonella Infections biology.protein bacteria Female Immunocompetence Antibody business medicine.drug |
| Zdroj: | Clinical and Experimental Immunology. 136:297-303 |
| ISSN: | 1365-2249 0009-9104 |
| Popis: | SUMMARYWe have developed a solid-phase enzyme-linked immunosorbent assay (ELISA) to study the vaccination responses to Vi capsular polysaccharide of Salmonella typhi (S. typhi Vi) vaccine. Purified S. typhi Vi polysaccharide was biotinylated and bound to streptavidin coated microtitre plates. Reproducibility was determined across a range of IgG antibody levels: mean interassay coefficients of variation (CVs) were |
| Databáze: | OpenAIRE |
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