Quantification and prognostic significance of interferon-γ secreting SARS-CoV-2 responsive T cells in hospitalized patients with acute COVID-19
| Autor: | Prashanth Patel, Andrea Tattersall, Daniel Pan, Joshua Nazareth, Jack Leach, Shirley Sze, Fleur Starcevic, Pranabashis Haldar, Adam Bellass, Caroline M. Williams, Jee Whang Kim, Andrea M. Cooper, Sara Assadi, Christopher A Martin, Manish Pareek, Adam Ahmed, James G Brosnan, Amandip Sahota, Michael R. Barer |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
medicine.medical_treatment T-Lymphocytes ACE2 HIV Infections Disease Spike protein medicine.disease_cause Antibodies Viral Antibodies Interferon-gamma Antigen medicine Humans Interferon gamma Continuous positive airway pressure Letter to the Editor Dexamethasone Coronavirus business.industry SARS-CoV-2 ELISPOT COVID-19 HIV medicine.disease Prognosis Immunity Humoral Infectious Diseases Immunology Spike Glycoprotein Coronavirus business Progressive disease medicine.drug |
| Zdroj: | The Journal of Infection |
| ISSN: | 1532-2742 |
| Popis: | Little is known about T-cell responses during acute coronavirus disease-2019 (COVID-19). We measured T-cell interferon gamma (IFN-{gamma}) responses to spike 1 (S1), spike 2 (S2), nucleocapsid (N) and membrane (M) SARS-CoV-2 antigens using the T-SPOT(R) Discovery SARS-CoV-2 assay, a proven EliSPOT technology, in 114 hospitalised adult COVID-19 patients and assessed their association with clinical disease phenotype. T-SPOT(R) Discovery SARS-CoV-2 responses were detectable within 2 days of a positive PCR and did not correlate with vaccination status or symptom duration. Higher responses to S1 protein associated with a higher symptom burden, and serum IL-6 levels. Despite treatment with dexamethasone this subgroup was also at greater risk of requiring continuous positive airway pressure (CPAP) in the days following sampling. Higher T-cell responses measured using T-SPOT(R) Discovery SARS-CoV-2 associate with progressive disease in acute COVID-19 disease and may have utility as a prognostic biomarker that should be evaluated in larger cohorts. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect