Recompleting the Caenorhabditis elegans genome
| Autor: | Lamia Wahba, Cheryl L. Smith, Mark L. Edgley, Karen L. Artiles, Jun Yoshimura, Ann E. Rougvie, Idan Gabdank, Erich M. Schwarz, Andrew Fire, Massa J. Shoura, Kazuki Ichikawa, Shinichi Morishita |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Resource
Systems biology Genomics Computational biology Genome Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Tandem repeat Genetics Animals Caenorhabditis elegans Caenorhabditis elegans Proteins Gene Genetics (clinical) 030304 developmental biology 0303 health sciences Genome Helminth biology Computational Biology High-Throughput Nucleotide Sequencing Reproducibility of Results Molecular Sequence Annotation biology.organism_classification Multicellular organism chemistry 030217 neurology & neurosurgery DNA |
| Zdroj: | Lab Anim (NY) |
| Popis: | Caenorhabditis elegans was the first multicellular eukaryotic genome sequenced to apparent completion. Although this assembly employed a standard C. elegans strain (N2), it used sequence data from several laboratories, with DNA propagated in bacteria and yeast. Thus, the N2 assembly has many differences from any C. elegans available today. To provide a more accurate C. elegans genome, we performed long-read assembly of VC2010, a modern strain derived from N2. Our VC2010 assembly has 99.98% identity to N2 but with an additional 1.8 Mb including tandem repeat expansions and genome duplications. For 116 structural discrepancies between N2 and VC2010, 97 structures matching VC2010 (84%) were also found in two outgroup strains, implying deficiencies in N2. Over 98% of N2 genes encoded unchanged products in VC2010; moreover, we predicted ≥53 new genes in VC2010. The recompleted genome of C. elegans should be a valuable resource for genetics, genomics, and systems biology. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|