A rapid method for the determination of artemisinin and its biosynthetic precursors in Artemisia annua L. crude extracts
| Autor: | Neil Sullivan, Alexei A. Lapkin, Susan E. Slade, Guy C. Barker, Lijiang Song, John O. Suberu |
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| Rok vydání: | 2013 |
| Předmět: |
Chromatography
biology Plant Extracts Elution Chemistry Clinical Biochemistry Artemisia annua Pharmaceutical Science Tandem mass spectrometry biology.organism_classification High-performance liquid chromatography Artemisinins Analytical Chemistry Standard curve Tandem Mass Spectrometry parasitic diseases Drug Discovery medicine Sample preparation Artemisinin Chromatography High Pressure Liquid Spectroscopy Dihydroartemisinic acid medicine.drug |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 84:269-277 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2013.06.025 |
| Popis: | A rapid high-pressure liquid chromatography (HPLC) tandem mass spectrometry (TQD) method for the determination of artemisinin, 9-epi-artemisinin, artemisitene, dihydroartemisinic acid, artemisinic acid and arteannuin B in Artemisia annua extracts is described. Detection and quantification of 9-epi-artemisinin in crude extracts are reported for the first time. In this method all six metabolites are resolved and eluted within 6 min with minimal sample preparation. A recovery of between 96.25% and 103.59% was obtained for all metabolites analysed and the standard curves were linear (r(2)0.99) over the concentration range of 0.15-10 μg mL(-1) for artemisinin, 9-epi-artemisinin, artemisitene and arteannuin B, and the range of 3.75-120 μg mL(-1) for dihydroartemisinic acid and artemisinic acid. All validation indices were satisfactory, showing the method to be robust, quick, sensitive and adequate for a range of applications including high throughput (HTP) analysis. |
| Databáze: | OpenAIRE |
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