Regulation of Chemokine Production via Oxidative Pathway in HeLa Cells
| Autor: | Shinichiro Kina, Feixin Liang, Hajime Sunakawa, Nao Sunagawa, Akira Matayoshi, Thongsavanh Phonaphonh, Toshiyuki Nakasone, Hiroyuki Takemoto, Shoko Makishi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
MAPK/ERK pathway
Chemokine Article Subject Iron Immunology Antimicrobial peptides Enzyme-Linked Immunosorbent Assay Biology HeLa Anti-Infective Agents lcsh:Pathology Humans Secretion Interleukin 8 Extracellular Signal-Regulated MAP Kinases chemistry.chemical_classification Reactive oxygen species Interleukin-8 Epithelial Cells Cell Biology Hydrogen Peroxide biology.organism_classification Molecular biology Oxygen chemistry Gene Expression Regulation biology.protein Signal transduction Chemokines Reactive Oxygen Species lcsh:RB1-214 Research Article HeLa Cells Phenanthrolines Signal Transduction |
| Zdroj: | Mediators of Inflammation Mediators of Inflammation, Vol 2009 (2009) |
| ISSN: | 1466-1861 0962-9351 |
| Popis: | Inflammation is associated with disease progression and, by largely unknown mechanisms, has been said to drive oncogenesis. At inflamed sites, neutrophils deploy a potent antimicrobial arsenal that includes proteinases, antimicrobial peptides, and ROS. Reactive oxygen species (ROSs) induce chemokines. In the present study, the concentrations of IL-8 in culture supernatants of HeLa cells treated with ROS were determined by enzyme-linked immunosorbent assay. We used 𝑜 -phenanthroline to deplete F e 2 + in order to investigate the mechanisms through which ROSs induce IL-8 secretion in our system. The iron chelator 𝑜 -phenanthroline effectively inhibited H 2 O 2 -induced ERK2 activation. Enzyme-linked immunosorbent assays showed that IL-8 protein secretion was elevated in ROS-treated HeLa cells. When F e 2 + was removed from these cells, IL-8 secretion was inhibited. Collectively, these results indicate that F e 2 + -mediated Erk pathway activation is an important signal transduction pathway in ROS-induced IL-8 secretion in epithelial cells. |
| Databáze: | OpenAIRE |
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