Parvovirus H-1 induces cytopathic effects in breast carcinoma-derived cultures

Autor: Dominique Stehelin, Nathalie Martin, Audrey Richard, Perrine Caillet-Fauquet, Patrick Devos, Marie-Christine Baranzelli, Ghaffar Muharram, Pierre Wizla, Agnes Begue, Emilie Le Rhun, Annie Roussel, Jacques Bonneterre, Ingrid Loison
Přispěvatelé: Institut de biologie de Lille - IBL (IBLI), Université de Lille, Sciences et Technologies-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Droit et Santé, Département de Sénologie, Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille] (UNICANCER/Lille), Université Lille Nord de France (COMUE)-UNICANCER-Université Lille Nord de France (COMUE)-UNICANCER, Département des Biostatistiques, Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Laboratoire d'Anatomo-cyto-pathologie, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), Université de Lille-UNICANCER-Université de Lille-UNICANCER, Service de Biostatistiques [CHRU Lille]
Rok vydání: 2009
Předmět:
Zdroj: Breast Cancer Research and Treatment
Breast Cancer Research and Treatment, Springer Verlag, 2009, 121 (1), pp.23-33. ⟨10.1007/s10549-009-0451-9⟩
Breast Cancer Research and Treatment, 2009, 121 (1), pp.23-33. ⟨10.1007/s10549-009-0451-9⟩
ISSN: 1573-7217
0167-6806
Popis: International audience; Parvovirus H-1 (H-1 PV) preferentially replicates in malignant cells resulting in their death by cytolysis. It has often been considered a potential candidate for use in novel anticancer therapy. To evaluate its potential in a model of natural tumors, we assayed in vitro the effect exerted by H-1 PV on short-term cultures derived from breast tumor samples freshly excised from patients. Our results show that H-1 PV effectively kills tumor-derived cells, whereas normal tissue-derived cells showed no H-1 PV-induced cytopathic effects (CPE). We also determined that the H-1 PV sensitivity (up to 67% sensitive cultures) is related with the quantities of virus assayed. We further examined the expression and phosphorylation state of the parvoviral nonstructural protein 1 (NS1), known to be associated with parvoviruses-induced CPE. Both appear to be impaired in normal tissue-derived cells and resistant cultures. Finally, we show that H-1 PV sensitivity in cultures correlates significantly with higher tumor grades (Nottingham combined histologic grade 2 or 3). This report confirms that H-1 PV can efficiently induce CPE in primary breast tumor cells in vitro. It identifies tumor characteristics representing potential criteria for recruiting patients for clinical evaluation of H-1 PV antitumor effects.
Databáze: OpenAIRE
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