Parvovirus H-1 induces cytopathic effects in breast carcinoma-derived cultures
Autor: | Dominique Stehelin, Nathalie Martin, Audrey Richard, Perrine Caillet-Fauquet, Patrick Devos, Marie-Christine Baranzelli, Ghaffar Muharram, Pierre Wizla, Agnes Begue, Emilie Le Rhun, Annie Roussel, Jacques Bonneterre, Ingrid Loison |
---|---|
Přispěvatelé: | Institut de biologie de Lille - IBL (IBLI), Université de Lille, Sciences et Technologies-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Droit et Santé, Département de Sénologie, Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille] (UNICANCER/Lille), Université Lille Nord de France (COMUE)-UNICANCER-Université Lille Nord de France (COMUE)-UNICANCER, Département des Biostatistiques, Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Laboratoire d'Anatomo-cyto-pathologie, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), Université de Lille-UNICANCER-Université de Lille-UNICANCER, Service de Biostatistiques [CHRU Lille] |
Rok vydání: | 2009 |
Předmět: |
H-1 parvovirus
Cancer Research Oncolytic virus Parvovirus H-1 Blotting Western Breast Neoplasms Mice SCID Biology Virus 03 medical and health sciences Mice Breast cancer 0302 clinical medicine medicine Animals Humans Cells Cultured 030304 developmental biology Oncolytic Virotherapy 0303 health sciences Parvovirus Short-term cultures biology.organism_classification medicine.disease Virology Xenograft Model Antitumor Assays In vitro 3. Good health Histopathology predictive factors Cytolysis Oncology 030220 oncology & carcinogenesis Cancer research Female Breast carcinoma HeLa Cells |
Zdroj: | Breast Cancer Research and Treatment Breast Cancer Research and Treatment, Springer Verlag, 2009, 121 (1), pp.23-33. ⟨10.1007/s10549-009-0451-9⟩ Breast Cancer Research and Treatment, 2009, 121 (1), pp.23-33. ⟨10.1007/s10549-009-0451-9⟩ |
ISSN: | 1573-7217 0167-6806 |
Popis: | International audience; Parvovirus H-1 (H-1 PV) preferentially replicates in malignant cells resulting in their death by cytolysis. It has often been considered a potential candidate for use in novel anticancer therapy. To evaluate its potential in a model of natural tumors, we assayed in vitro the effect exerted by H-1 PV on short-term cultures derived from breast tumor samples freshly excised from patients. Our results show that H-1 PV effectively kills tumor-derived cells, whereas normal tissue-derived cells showed no H-1 PV-induced cytopathic effects (CPE). We also determined that the H-1 PV sensitivity (up to 67% sensitive cultures) is related with the quantities of virus assayed. We further examined the expression and phosphorylation state of the parvoviral nonstructural protein 1 (NS1), known to be associated with parvoviruses-induced CPE. Both appear to be impaired in normal tissue-derived cells and resistant cultures. Finally, we show that H-1 PV sensitivity in cultures correlates significantly with higher tumor grades (Nottingham combined histologic grade 2 or 3). This report confirms that H-1 PV can efficiently induce CPE in primary breast tumor cells in vitro. It identifies tumor characteristics representing potential criteria for recruiting patients for clinical evaluation of H-1 PV antitumor effects. |
Databáze: | OpenAIRE |
Externí odkaz: |