Effects of interleukin-8 and Helicobacter pylori on histamine release from isolated canine gastric mucosal mast cells
| Autor: | Shoki Ro, Yasutaka Minagawa, Takashi Nakamura, Mitsuhito Koyanagi, Koji Yakabi, Takashi Arimura, Yoichi Uehigashi |
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| Rok vydání: | 2002 |
| Předmět: |
Stomach Diseases
Inflammation In Vitro Techniques Histamine Release Helicobacter Infections chemistry.chemical_compound Dogs Bacterial Proteins medicine Gastric mucosa Concanavalin A Animals Interleukin 8 Mast Cells Enterochromaffin-like cell Cells Cultured biology Helicobacter pylori Interleukin-8 Gastroenterology Radioimmunoassay biology.organism_classification Molecular biology medicine.anatomical_structure chemistry Gastric Mucosa Immunology biology.protein medicine.symptom Histamine |
| Zdroj: | Journal of gastroenterology. 37(1) |
| ISSN: | 0944-1174 |
| Popis: | In recent studies, the involvement of mast cells in the pathogenesis of Helicobacter pylori infection was suggested. In the present study, using isolated canine gastric mucosal mast cells, we undertook to elucidate the effects of interleukin-8 (IL-8) and H. pylori on histamine release from these cells. Methods. Enriched canine gastric mucosal mast cells (50% target cells) were incubated in Hanks medium with IL-8, or water extract or sonicate of H. pylori for 15 min at 37°C. The content of histamine in the supernatants and the cell pellets after centrifugation was assayed with a histamine radioimmunoassay (RIA) kit. Results. IL-8 (50 ng/ml) and concanavalin A (20 μg/ml) significantly increased histamine release from enriched gastric mucosal mast cells. Dose-dependent stimulation of histamine release by IL-8 (5–50 ng/ml) was also seen. Water extract and sonicate of H. pylori (108 bacteria) increased histamine release from mast cells. A concentration-dependent stimulation of histamine release by water extract or sonicate was also seen. The maximal response of histamine release was seen at the highest concentration of the water extract or sonicate. Conclusions. The results indicated that IL-8 and H. pylori had stimulatory effects on histamine release from canine gastric mucosal mast cells. The results imply that IL-8 and soluble factors of H. pylori may accelerate inflammation of the gastric mucosa via histamine release from mast cells. |
| Databáze: | OpenAIRE |
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