Is there any role of epithelial to mesenchymal transition in the pathogenesis of contrast nephropathy?

Autor: Garip Bekfilavioglu, Ali Okuyucu, Eser Yenen, Abdulkerim Bedir, Osman Salis, Hayriye Sayarlioglu, Coskun Kaya
Přispěvatelé: OMÜ
Rok vydání: 2016
Předmět:
0301 basic medicine
Pathology
medicine.medical_specialty
iopromide
Epithelial-Mesenchymal Transition
Iohexol
medicine.medical_treatment
tubular epithelial cells culture
030232 urology & nephrology
Contrast Media
Connective tissue
Protein Serine-Threonine Kinases
Critical Care and Intensive Care Medicine
Collagen Type I
Cell Line
Immediate-Early Proteins
Kidney Tubules
Proximal
03 medical and health sciences
0302 clinical medicine
HK-2 cells
Humans
Medicine
Epithelial–mesenchymal transition
business.industry
Growth factor
Mesenchymal stem cell
Connective Tissue Growth Factor
Iopromide
Cell Differentiation
General Medicine
Contrast nephropathy
Collagen Type I
alpha 1 Chain
CTGF
Collagen
type I
alpha 1
030104 developmental biology
medicine.anatomical_structure
Nephrology
Cell culture
Cancer research
Kidney Diseases
epithelial-to-mesenchymal transition
Snail Family Transcription Factors
business
medicine.drug
Zdroj: Renal Failure. 38:1249-1255
ISSN: 1525-6049
0886-022X
DOI: 10.1080/0886022x.2016.1209381
Popis: WOS: 000383902000015 PubMed: 27435174 Aim: Contrast medium-induced nephropathy is one of the major complications of intravenous contrast medium use. But its pathogenesis is unclear. Epithelial mesenchymal transition (EMT) is defined as the transformation of the primer epithelial cells to mesenchymal cells. EMT in tubular cells might cause tubulointerstitial damage. In this study, we investigated whether or not EMT has a role in radiocontrast-induced nephropathy. Radiocontrast medium might be triggering reversible EMT via serum and glucocorticoid-regulated kinase 1 (SGK 1). We investigated the effect of different concentrations of the contrast agent iopromide on human proximal tubule cell (HK-2) culture by measuring the level of SGK1, snail family zinc finger 1 (SNAIL1), connective tissue growth factor (CTGF), and collagen type I alpha 1 (COL1A1).Methods: We conducted a scratch assay and qPCR. HK-2 cells were cultured in the petri dishes/flasks and starved with serum-free medium. The 40, 20, and 10mg/mL doses of iopromide were administrated to cells. The scratches were photographed immediately and again at the 20th hour. The levels of gene expression of SGK1, SNAIL1, CTGF, and COL1A1 were measured using the real-time qPCR system at the end of the 24th hour.Results: Iopromide caused the breaking of intercellular connections, the disappearance of the cobblestone appearance of cells, and the migration of cells at the 20th hour in the scratch assay. It also increased the expression of SGK1, SNAIL1, CTGF, and COL1A1 genes.Conclusion: Our study concluded that certain important markers of EMT increase in different concentrations of the contrast agent. High osmolality might trigger EMT. The relationship between contrast agent and EMT has not been defined before. Further in vivo and in vitro studies are required. research foundation of Ondokuz Mayis University [PYO.TIP.1901.13.046] This study was supported by the research foundation of Ondokuz Mayis University [PYO.TIP.1901.13.046].
Databáze: OpenAIRE
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