Design and performance of a second-generation surface-induced dissociation cell for Fourier transform ion cyclotron resonance mass spectrometry of native protein complexes
| Autor: | Alyssa Q. Stiving, Erin M. Panczyk, Árpád Somogyi, Joshua D. Gilbert, Vicki H. Wysocki, Desmond Allen Kaplan, Dalton T. Snyder |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Streptavidin
Fourier Analysis Chemistry Surface Properties 010401 analytical chemistry Analytical chemistry Proteins Protonation Equipment Design Cyclotrons 010402 general chemistry 01 natural sciences Dissociation (chemistry) Fourier transform ion cyclotron resonance Article Mass Spectrometry 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Tetramer Electrode Molecule Ion trap Electrodes |
| Popis: | A second-generation ("Gen 2") device capable of surface-induced dissociation (SID) and collision-induced dissociation (CID) for Fourier transform ion cyclotron resonance mass spectrometry of protein complexes has been designed, simulated, fabricated, and experimentally compared to a first-generation device ("Gen 1"). The primary goals of the redesign were to (1) simplify SID by reducing the number of electrodes, (2) increase CID and SID sensitivity by lengthening the collision cell, and (3) increase the mass range of the device for analysis of larger multimeric proteins, all while maintaining the normal instrument configuration and operation. Compared to Gen 1, Gen 2 exhibits an approximately 10× increase in sensitivity in flythrough mode, 7× increase in CID sensitivity for protonated leucine enkephalin (m/z 556), and 14× increase of CID sensitivity of 53 kDa streptavidin tetramer. It also approximately doubles the useful mass range (from m/z 8000 to m/z 15 000) using a rectilinear ion trap with a smaller inscribed radius or triples it (to m/z 22 000) using a hexapole collision cell and yields a 3-10× increase in SID sensitivity. We demonstrate the increased mass range and sensitivity on a variety of model molecules spanning nearly 3 orders of magnitude in absolute mass and present examples where the high resolution of the FT-ICR is advantageous for deconvoluting overlapping SID fragments. |
| Databáze: | OpenAIRE |
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