LINE-1 retrotransposon encoded ORF1p expression and promoter methylation in oral squamous cell carcinoma: a pilot study
| Autor: | Koel Mukherjee, Manash Biswas, Debpali Sur, Prabhat Kumar Mandal, Sofia Pilli, Ramasare Prasad, Savita Budania, Shilpi Saxena, Jitendra Nangal |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
Population Pilot Projects Retrotransposon Biology 03 medical and health sciences 0302 clinical medicine Promoter methylation Biomarkers Tumor Genetics medicine Humans Basal cell Promoter Regions Genetic education Molecular Biology education.field_of_study Proteins Cancer DNA Methylation Prognosis medicine.disease Long Interspersed Nucleotide Elements 030220 oncology & carcinogenesis Carcinoma Squamous Cell Cancer research biology.protein Immunohistochemistry Mouth Neoplasms Human genome Antibody |
| Zdroj: | Cancer Genetics. 244:21-29 |
| ISSN: | 2210-7762 |
| DOI: | 10.1016/j.cancergen.2020.01.050 |
| Popis: | Oral squamous cell carcinoma (OSCC) is highly predominant in India due to excessive use of tobacco. Here we investigated Long INterpersed Element 1 (LINE or L1) retrotransposon activity in OSCC samples in the same population. There are almost 500,000 copies of L1 occupied around 30% of the human genome. Although most of them are inactive, around 150–200 copies are actively jumping in a human genome. L1 encodes two proteins designated as ORF1p and ORF2p and expression of both proteins are critical for the process of retrotransposition. Here we have analyzed L1 ORF1p expression in a small cohort (n = 15) of paired cancer-normal tissues obtained from operated oral cancer patients. Immunohistochemistry (IHC) with the human ORF1 antibody showed the presence of ORF1p in almost 60% cancer samples, and few of them also showed aberrant p53 expression. Investigating L1 promoter methylation status, showed certain trends towards hypomethylation of the L1 promoter in cancer tissues compared to its normal counterpart. Our data raise the possibility that L1ORF1p expression might have some role in the onset and progression of this particular type of cancer. |
| Databáze: | OpenAIRE |
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