Slug Is a Predictor of Poor Prognosis in Esophageal Squamous Cell Carcinoma Patients
| Autor: | Ranju Ralhan, Anoop Saraya, Rinu Sharma, Paul G. Walfish, Md. Raghibul Hasan, Tushar K. Chattopadhyay, Shyam S. Chauhan, Siddartha DattaGupta |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Pathology Esophageal Neoplasms Slug lcsh:Medicine Kaplan-Meier Estimate In Vitro Techniques Biology Gastroenterology Disease-Free Survival 03 medical and health sciences 0302 clinical medicine Internal medicine Biomarkers Tumor medicine Humans Clinical significance lcsh:Science neoplasms Survival analysis 030304 developmental biology 0303 health sciences Multidisciplinary Proportional hazards model lcsh:R Histology Esophageal cancer Prognosis medicine.disease biology.organism_classification Immunohistochemistry digestive system diseases 3. Good health Dysplasia 030220 oncology & carcinogenesis Carcinoma Squamous Cell lcsh:Q Female Esophageal Squamous Cell Carcinoma Snail Family Transcription Factors Transcription Factors Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 12, p e82846 (2013) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0082846 |
| Popis: | Background Slug, a regulator of epithelial mesenchymal transition, was identified to be differentially expressed in esophageal squamous cell carcinoma (ESCC) using cDNA microarrays by our laboratory. This study aimed to determine the clinical significance of Slug overexpression in ESCC and determine its correlation with clinicopathological parameters and disease prognosis for ESCC patients. Methods Immunohistochemical analysis of Slug expression was carried out in archived tissue sections from 91 ESCCs, 61 dysplastic and 47 histologically normal esophageal tissues. Slug immunopositivity in epithelial cells was correlated with clinicopathological parameters and disease prognosis over up to 7.5 years for ESCC patients. Results Increased expression of Slug was observed in esophageal dysplasia [cytoplasmic, 24/61 (39.3%) cases, p = 0.001, odd’s ratio (OR) = 4.7; nuclear, 11/61 (18%) cases, p < 0.001, OR = 1.36] in comparison with normal esophageal tissues. The Slug expression was further increased in ESCCs [cytoplasmic, 64/91 (70.3%) p < 0.001, OR = 10.0; nuclear, 27/91 (29.7%) p < 0.001, OR = 1.42]. Kaplan Meier survival analysis showed significant association of nuclear Slug accumulation with reduced disease free survival of ESCC patients (median disease free survival (DFS) = 6 months, as compared to those that did not show overexpression, DFS = 18 months; p = 0.006). In multivariate Cox regression analysis nuclear Slug expression [p= 0.005, Hazard’s ratio (HR) = 2.269, 95% CI = 1.289 - 3.996] emerged as the most significant independent predictor of poor prognosis for ESCC patients. Conclusions Alterations in Slug expression occur in early stages of development of ESCC and are sustained during disease progression. Slug may serve as a diagnostic biomarker and as a predictor of poor disease prognosis to identify ESCC patients that are likely to show recurrence of the disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|