Allogeneic Bone Marrow Mesenchymal Stem Cell Transplantation in Tooth Extractions Sites Ameliorates the Incidence of Osteonecrotic Jaw-Like Lesions in Zoledronic Acid-Treated Rats
| Autor: | David García-Bernal, Marta Vallés-Bergadá, Julia Guerrero-Gironés, R. E. Oñate-Sánchez, Beatriz Revilla-Nuin, Carlos Martinez, Francisco Javier Rodríguez-Lozano, Mar Gonzálvez-García, José M. Moraleda |
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| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Allogeneic transplantation medicine.medical_treatment lcsh:Medicine MRONJ Article zoledronic acid 03 medical and health sciences jaw 0302 clinical medicine Medicine Saline business.industry lcsh:R osteonecrosis Mesenchymal stem cell bone marrow mesenchymal stem cells 030206 dentistry General Medicine Bisphosphonate medicine.disease Pathophysiology Transplantation Zoledronic acid 030220 oncology & carcinogenesis business Osteonecrosis of the jaw medicine.drug |
| Zdroj: | Journal of Clinical Medicine Volume 9 Issue 6 Journal of Clinical Medicine, Vol 9, Iss 1649, p 1649 (2020) |
| ISSN: | 2077-0383 |
| Popis: | Medication-related osteonecrosis of the jaw (MRONJ) is defined as the exposed necrotic bone involving the maxillofacial structures in bisphosphonate treated patients, and the pathophysiology of this disease remains unclear. The aim of this study was to assess the effects of the allogeneic transplantation of bone marrow-derived mesenchymal stem cells (BM-MSCs) in a model of Wistar mice with induced MRONJ disease. BM-MSCs from five male Wistar rats were characterized and cultured on &beta tricalcium phosphate (&beta TCP) granules. Thirty female Wistar rats were injected intraperitoneally with zoledronic acid and afterwards upper jaw molars were extracted. The animals were randomized to receive: Group 1: 1 × 106 BM-MSCs/&beta TCP construct in the alveolar socket and Group 2: Saline solution/&beta TCP construct. A clinical and histological analysis was performed. Nested polymerase chain reaction (PCR) was assessed to verify the presence of transplanted male rat cells in the female recipient jaws. Clinical and histological findings evidenced that none of the animals in Group 1 exhibited uncovered sockets or bone exposure associated to MRONJ, whereas we detected 33% of MRONJ cases in Group 2. In addition, male rat cells were detected in the maxillae site four weeks after transplantation in the BM-MSCs-group. Allogeneic BM-MSCs in extractions sites ameliorates MRONJ incidence in zoledronic acid-treated rats compared to non-MSC treatments. |
| Databáze: | OpenAIRE |
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