A new pH sensor localized in the Golgi apparatus of Saccharomyces cerevisiae reveals unexpected roles of Vph1p and Stv1p isoforms
| Autor: | Olga Zimmermannova, Pierre Morsomme, Antoine Deschamps, Anne-Sophie Colinet, Hana Sychrová |
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| Přispěvatelé: | UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Gene isoform Vacuolar Proton-Translocating ATPases Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Ph control Golgi Apparatus lcsh:Medicine Biosensing Techniques Microbiology Article 03 medical and health sciences symbols.namesake Golgi lcsh:Science Sensors and probes Secretory pathway Organelles Multidisciplinary 030102 biochemistry & molecular biology biology Chemistry lcsh:R Fungi Wild type Chemical Engineering Hydrogen-Ion Concentration Golgi apparatus biology.organism_classification Cell biology Isoenzymes Cytosol 030104 developmental biology symbols lcsh:Q Lumen (unit) |
| Zdroj: | Scientific reports, Vol. 10, no.1, p. 1881 (2020) Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) Scientific Reports |
| Popis: | The gradual acidification of the secretory pathway is conserved and extremely important for eukaryotic cells, but until now there was no pH sensor available to monitor the pH of the early Golgi apparatus in Saccharomyces cerevisiae. Therefore, we developed a pHluorin-based sensor for in vivo measurements in the lumen of the Golgi. By using this new tool we show that the cis- and medial-Golgi pH is equal to 6.6–6.7 in wild type cells during exponential phase. As expected, V-ATPase inactivation results in a near neutral Golgi pH. We also uncover that surprisingly Vph1p isoform of the V-ATPase is prevalent to Stv1p for Golgi acidification. Additionally, we observe that during changes of the cytosolic pH, the Golgi pH is kept relatively stable, mainly thanks to the V-ATPase. Eventually, this new probe will allow to better understand the mechanisms involved in the acidification and the pH control within the secretory pathway. |
| Databáze: | OpenAIRE |
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