Spectroscopic and AFM characterization of polypeptide-surface interactions: Controls and lipid quantitative analyses
| Autor: | Øyvind Halskau, Hanzhen Wen, Øyvind Strømland, Samuel Furse, Morten L. Govasli, Ørjan S. Handegård |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Circular dichroism Conformational change PC Phosphatidylcholine Lipid bilayers DPX p-Xylene-Bis-Pyridinium Bromide PS Phosphatidylserine NMR Nuclear Magnetic Resonance lcsh:Computer applications to medicine. Medical informatics FRET Förster Resonance Energy Transfer Solid-supported bilayers Fluorescence spectroscopy Fluorescence 03 medical and health sciences Quantitiative 31P NMR EYPC egg yolk phosphatidylcholine Spin-coating Lipid bilayer lcsh:Science (General) Data Article Multidisciplinary ANTS 8-Aminonaphthalene-1 3 6-Trisulfonic Acid Disodium Salt 030102 biochemistry & molecular biology CD Circular Dichroism PBPS porcine brain phosphatidylserine Chemistry CUBO solvent Culeddu-Bosco solvent Vesicle Polypeptide aggregation DLS Dynamic Light Scattering PA Phosphatidic Acid PC-plas Phosphatidylcholine plasmalogen Chemical degradation Crystallography 030104 developmental biology Förster resonance energy transfer Membrane AFM Atomic Force Microscopy SLB Solid-supported Lipid Bilayers PS-plas Phosphatidylserine plasmalogen lcsh:R858-859.7 LUV Large Unilamellar Vesicles lcsh:Q1-390 |
| Zdroj: | Data in Brief Data in Brief, Vol 12, Iss C, Pp 113-122 (2017) |
| ISSN: | 2352-3409 |
| Popis: | This article is related to http://dx.doi.org/10.1016/j.bbamem.2017.01.005 (O. Stromland, O.S. Handegard, M.L. Govasli, H. Wen, O. Halskau, 2017) [1]. In protein and polypeptide-membrane interaction studies, negatively charged lipids are often used as they are a known driver for membrane interaction. When using fluorescence spectroscopy and CD as indicators of polypeptide binding and conformational change, respectively, the effect of zwitterionic lipids only should be documented. The present data documents several aspects of how two engineered polypeptides (A-Cage-C and A-Lnk-C) derived from the membrane associating protein alpha-Lactalbumin affects and are affected by the presence of zwitterionic bilayers in the form of vesicles. We here document the behavior or the Cage and Lnk segments with respect to membrane interaction and their residual fold, using intrinsic tryptophan fluorescence assays. This data description also documents the coverage of solid-supported bilayers prepared by spin-coating mica using binary lipid mixes, a necessary step to ensure that AFM is performed on areas that are covered by lipid bilayers when performing experiments. Uncovered patches are detectable by both force curve measurements and height measurements. We tested naked mica׳s ability to cause aggregation as seen by AFM, and found this to be low compared to preparations containing negatively charged lipids. Work with lipids also carries the risk of chemical degradation taking place during vesicles preparation or other handling of the lipids. We therefor use 31P NMR to quantify the head-group content of commonly used commercial extracts before and after a standard protocol for vesicle production is applied. |
| Databáze: | OpenAIRE |
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