Lack of expression of TUBB3 characterizes both BCL2-positive and BCL2-negative follicular lymphoma
| Autor: | Francesco Erdini, Marco Chilosi, Alberto Zamò, Giovanni Malerba |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty BCL2 Follicular lymphoma follicular hyperplasia follicular lymphoma TUBB3 Biology Pathology and Forensic Medicine Diagnosis Differential immune system diseases Tubulin hemic and lymphatic diseases Follicular phase medicine Biomarkers Tumor Humans music neoplasms Lymphoma Follicular Aged Retrospective Studies music.instrument Hyperplasia Germinal center Middle Aged medicine.disease Germinal Center Follicular hyperplasia Immunohistochemistry Staining BCL2 follicular hyperplasia follicular lymphoma TUBB3 Proto-Oncogene Proteins c-bcl-2 Female biological phenomena cell phenomena and immunity Positive staining |
| Zdroj: | Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. 27(6) |
| ISSN: | 1530-0285 |
| Popis: | Follicular lymphoma is characterized by aberrant BCL2 expression, a feature that is exploited for diagnostic purposes. However, a certain percentage of follicular lymphomas might be BCL2-negative by immunohistochemistry, increasing the difficulties in differentiating them from follicular hyperplasia. The expression of TUBB3 has been recently reported as negative in a small series of follicular lymphomas. We have therefore tested a larger series, including 61 BCL2-positive and 25 BCL2-negative cases, and compared them with 61 reactive lymphoid tissues. First, a subjective score of TUBB3 staining was applied, showing that it was consistently positive in reactive germinal centers, while most follicular lymphomas were negative; in fact, only 10/61 (16%) BCL2-positive and 1/25 (4%) BCL2-negative cases showed a positive staining for TUBB3, while 58/61 (95%) of tissues with follicular hyperplasia were positive. The application of a standardized scoring system to a large number of follicles, based on virtual slides, demonstrated that reactive lymphoid tissues had a significantly higher number of TUBB3-positive follicles both compared with BCL2-positive cases and to BCL2-negative cases. Our data support the use of TUBB3 staining in differentiating follicular lymphoma, including BCL2-negative cases, from follicular hyperplasia. |
| Databáze: | OpenAIRE |
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