Adhesion molecules and microvascular changes in the nonobese diabetic (NOD) mouse pancreas. An NO-inhibitor (L-NAME) is unable to block adhesion inflammation-induced activation
| Autor: | Francesco Aurelio Pisanti, Konrad Federlin, Gianpaolo Papaccio, Thomas Linn, Michael V.G. Latronico |
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| Přispěvatelé: | Papaccio, Gianpaolo, Latronico, Mv, Pisanti, Fa, Federlin, K, Linn, T. |
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Immunology Inflammation Biology Pathogenesis Islets of Langerhans Mice Mice Inbred NOD Internal medicine medicine Cell Adhesion Immunology and Allergy Animals Enzyme Inhibitors Pancreas Cells Cultured NOD mice Cell adhesion molecule Pancreatic islets Macrophages Microcirculation Histocompatibility Antigens Class II Macrophage Activation Intercellular Adhesion Molecule-1 Immunohistochemistry Endothelial stem cell Endocrinology medicine.anatomical_structure NG-Nitroarginine Methyl Ester Cancer research Endothelium Vascular medicine.symptom Nitric Oxide Synthase Blood vessel |
| Zdroj: | Scopus-Elsevier |
| Popis: | The aim of the present study was to investigate the immunoreactivity of pancreatic microvasculature with emphasis on the adhesion molecule expression in NOD mice at a very early stage and after the start of infiltration, before the onset of the diabetic disease. Immunoreactivity for Ia-b, BM8 (mouse macrophages) and inter-cellular-adhesion-molecule-1 (ICAM-1) molecules in untreated control mice and in animals treated using an inhibitor of nitric oxide (NO) formation (L-arginine analogue), as well as islet culture, nitrite assay and ultrastructural studies were performed. Results showed that Ia-b and ICAM-1 immunoreactivities on endothelia are a very early phenomenon and that pancreatic blood vessels and, in particular, some peri-islet venules, as well as several venules of the exocrine parenchyma, undergo significant morphological changes. Several endothelial cells of both peri-islet and extra-islet compartments, often showed Ia-b and ICAM-1 immunoreactivities, demonstrating that these cells are important for the adhesion processes taking place during early autoimmune inflammation. Inhibition of NO formation does not significantly affect ICAM-1 and Ia-b immunoreactivity both in vivo and in vitro, BM8 immunoreactive cells were considerably less in number although these were detected either around islets or along pancreatic septa, but rarely within the epithelial layer. |
| Databáze: | OpenAIRE |
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