Host microbiota modulates development of social preference in mice

Autor:	Rochellys Diaz Heijtz, Henrike Raith, Hans Forssberg, Tim Arentsen, Yu Qian
Přispěvatelé:	Foundation Olle Engkvist Byggmästare, Swedish Brain Foundation, Swedish Research Council, Strategic Neuroscience Programme at Karolinska Institutet.
Jazyk:	angličtina
Rok vydání:	2015
Předmět:	medicine.medical_specialty brain development Biology Gut flora Social identity approach Amygdala lcsh:Microbial ecology Exon Neurotrophic factors germ-free mice Internal medicine Gene expression medicine Gene synaptic plasticity genes Genetics gene expression amygdala BDNF biology.organism_classification medicine.anatomical_structure Endocrinology Synaptic plasticity lcsh:QR100-130 Original Article
Zdroj:	Microbial Ecology in Health and Disease, Vol 26, Iss 0, Pp 1-8 (2015) Microbial Ecology in Health and Disease; Vol 26 (2015) Microbial Ecology in Health and Disease
ISSN:	1651-2235
Popis:	Background : Mounting evidence indicates that the indigenous gut microbiota exerts long-lasting programming effects on brain function and behaviour. Objective : In this study, we used the germ-free (GF) mouse model, devoid of any microbiota throughout development, to assess the influence of the indigenous microbiota on social preference and repetitive behaviours (e.g. self-grooming). Methods and results : Using the three-chambered social approach task, we demonstrate that when adult GF mice were given a choice to spend time with a novel mouse or object, they spent significantly more time sniffing and interacting with the stimulus mouse compared to conventionally raised mice (specific pathogen-free, SPF). Time spent in repetitive self-grooming behaviour, however, did not differ between GF and SPF mice. Real-time PCR–based gene expression analysis of the amygdala, a key region that is part of the social brain network, revealed a significant reduction in the mRNA levels of total brain-derived neurotrophic factor (BDNF), BDNF exon I-, IV-, VI-, IX-containing transcripts, and NGFI-A (a signalling molecule downstream of BDNF) in GF mice compared to SPF mice. Conclusion : These results suggest that differential regulation of BDNF exon transcripts in the amygdala by the indigenous microbes may contribute to the altered social development of GF mice. Keywords: brain development; germ-free mice; gene expression; synaptic plasticity genes; amygdala; BDNF (Published: 15 December 2015) Citation: Microbial Ecology in Health & Disease 2015, 26: 29719 - http://dx.doi.org/10.3402/mehd.v26.29719
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::815fa56ef79e93259fbd10b583f5cb78 http://www.microbecolhealthdis.net/index.php/mehd/article/view/29719/44055 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.