Processing-Independent CRISPR RNAs Limit Natural Transformation in Neisseria meningitidis
| Autor: | Erik J. Sontheimer, Yan Zhang, Christoph Schoen, Biju Joseph Ampattu, Carl W. Gunderson, Jörg Vogel, H. Steven Seifert, Nadja Heidrich |
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| Rok vydání: | 2013 |
| Předmět: |
Ribonuclease III
Transcription Genetic Neisseria meningitidis Article CRISPR Spacers Plasmid Bacterial Proteins Sequence Homology Nucleic Acid Humans CRISPR RNA Processing Post-Transcriptional Promoter Regions Genetic Molecular Biology Gene Genetics Trans-activating crRNA CRISPR interference Base Sequence Models Genetic Virulence biology Inverted Repeat Sequences RNA Cell Biology Meningococcal Infections RNA Bacterial Genes Bacterial Host-Pathogen Interactions biology.protein Transformation Bacterial |
| Zdroj: | Molecular Cell. 50:488-503 |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/j.molcel.2013.05.001 |
| Popis: | CRISPR interference confers adaptive, sequence-based immunity against viruses and plasmids and is specified by CRISPR RNAs (crRNAs) that are transcribed and processed from spacer-repeat units. Pre-crRNA processing is essential for CRISPR interference in all systems studied thus far. Here, our studies of crRNA biogenesis and CRISPR interference in naturally competent Neisseria spp. reveal a unique crRNA maturation pathway in which crRNAs are transcribed from promoters that are embedded within each repeat, yielding crRNA 5' ends formed by transcription and not by processing. Although crRNA 3' end formation involves RNase III and trans-encoded tracrRNA, as in other type II CRISPR systems, this processing is dispensable for interference. The meningococcal pathway is the most streamlined CRISPR/Cas system characterized to date. Endogenous CRISPR spacers limit natural transformation, which is the primary source of genetic variation that contributes to immune evasion, antibiotic resistance, and virulence in the human pathogen N. meningitidis. |
| Databáze: | OpenAIRE |
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