Systematic evaluation of self-adjuvanting lipopeptide nano-vaccine platforms for the induction of potent CD8(+) T-cell responses
| Autor: | Istvan Toth, Penny L. Groves, Salwa Aljohani, Sharareh Eskandari, Pavla Simerska, Rachel J. Stephenson, Simon H. Apte, Denise L. Doolan |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Biomedical Engineering Medicine (miscellaneous) Bioengineering Development Biology CD8-Positive T-Lymphocytes Cancer Vaccines Epitope 03 medical and health sciences chemistry.chemical_compound Lipopeptides Mice Nanocapsules In vivo Cytotoxic T cell Animals General Materials Science Polylysine Particle Size Effector Lipopeptide Lauric Acids Dendritic cell Neoplasms Experimental Mice Inbred C57BL 030104 developmental biology Treatment Outcome chemistry Biochemistry Peptide vaccine lipids (amino acids peptides and proteins) |
| Zdroj: | Nanomedicine (London, England). 11(2) |
| ISSN: | 1748-6963 |
| Popis: | Aim: Systematically evaluate lipid core peptide vaccine delivery platforms to identify core features promoting strong CD8+ T-cell responses. Materials & methods: Three different self-adjuvanting lipid core peptide nanovaccines each comprising four copies of the dominant ovalbumin CD8+ T-cell epitope and varying in the utilization of a polylysine or glucose core with 2-amino-hexadecanoic acid (C16) or 2-amino-dodecanoic acid (C12) lipids were synthesized. Vaccines were tested for ability to induce CD8+ T-cell responses and inhibit tumor growth in vivo. Results: The construct utilizing C12 lipids and polylysine core induced very robust effector T cells shown to have in vivo effector capability as demonstrated by in vivo cytotoxicity and ability to inhibit tumor growth as well as modulation of dendritic cell activation. Conclusion: The C12 polylysine platform was an effective configuration for induction of potent CD8+ T-cell responses. |
| Databáze: | OpenAIRE |
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