Reprogramming mechanisms influence the maturation of hematopoietic progenitors from human pluripotent stem cells
| Autor: | Kye Seong Kim, Hye Ryun Kim, Hye Ryeon Heo, Woo Jin Kim, Jeong Eun Lee, Dong Ryul Lee, Young Gie Chung, Haengseok Song, Se Ran Yang, Seok Ho Hong, Taehoon Chun |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pluripotent Stem Cells Cancer Research Nuclear Transfer Techniques Transcription Genetic Immunology Human Embryonic Stem Cells Induced Pluripotent Stem Cells Biology Article Cell Line Transcriptome Colony-Forming Units Assay 03 medical and health sciences Cellular and Molecular Neuroscience Humans Progenitor cell lcsh:QH573-671 Induced pluripotent stem cell Cell Proliferation lcsh:Cytology Cell Differentiation Cell Biology DNA Methylation Cellular Reprogramming Hematopoietic Stem Cells Cell biology Haematopoiesis 030104 developmental biology Alveolar Epithelial Cells DNA methylation Somatic cell nuclear transfer Stem cell Reprogramming Germ Layers |
| Zdroj: | Cell Death and Disease, Vol 9, Iss 11, Pp 1-13 (2018) Cell Death & Disease |
| ISSN: | 2041-4889 |
| Popis: | Somatic cell nuclear transfer (SCNT) or the forced expression of transcription factors can be used to generate autologous pluripotent stem cells (PSCs). Although transcriptomic and epigenomic comparisons of isogenic human NT-embryonic stem cells (NT-ESCs) and induced PSCs (iPSCs) in the undifferentiated state have been reported, their functional similarities and differentiation potentials have not been fully elucidated. Our study showed that NT-ESCs and iPSCs derived from the same donors generally displayed similar in vitro commitment capacity toward three germ layer lineages as well as proliferative activity and clonogenic capacity. However, the maturation capacity of NT-ESC-derived hematopoietic progenitors was significantly greater than the corresponding capacity of isogenic iPSC-derived progenitors. Additionally, donor-dependent variations in hematopoietic specification and commitment capacity were observed. Transcriptome and methylome analyses in undifferentiated NT-ESCs and iPSCs revealed a set of genes that may influence variations in hematopoietic commitment and maturation between PSC lines derived using different reprogramming methods. Here, we suggest that genetically identical iPSCs and NT-ESCs could be functionally unequal due to differential transcription and methylation levels acquired during reprogramming. Our proof-of-concept study indicates that reprogramming mechanisms and genetic background could contribute to diverse functionalities between PSCs. |
| Databáze: | OpenAIRE |
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