Spatiotemporal expression of the cocaine- and amphetamine-regulated transcript-like (cart-like) gene during zebrafish embryogenesis
| Autor: | Rie Ohga, Kanoko Yanagi, Kiyohito Taimatsu, Takaaki Mori, Hitoshi Morita, Hiroaki Suzuki, Atsuo Kawahara |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
animal structures Embryo Nonmammalian Neurogenesis Embryonic Development Sequence Homology Nerve Tissue Proteins In situ hybridization Cocaine and amphetamine regulated transcript 03 medical and health sciences Spatio-Temporal Analysis immune system diseases Genetics Animals Amino Acid Sequence Molecular Biology Gene Zebrafish Phylogeny biology Embryogenesis virus diseases Gene Expression Regulation Developmental Gastrula Blastula Zebrafish Proteins biology.organism_classification Cell biology 030104 developmental biology nervous system embryonic structures Pharyngula Neural development Developmental Biology |
| Zdroj: | Gene expression patterns : GEP. 30 |
| ISSN: | 1872-7298 |
| Popis: | The cocaine- and amphetamine-regulated transcript (CART) genes are involved in the neural regulation of energy homeostasis; however, their developmental expressions and functions are not fully understood in vertebrates. We have identified a novel zebrafish cart-like gene that encodes a protein of 105 amino acids possessing sequence similarity to zebrafish and mammalian CART proteins. RT-PCR analysis revealed that the cart-like transcripts were maternally supplied and gradually decreased during the cleavage, blastula and gastrula stages; then, transcripts subsequently reaccumulated at the segmentation, pharyngula and hatching stages. Based on a whole-mount in situ hybridization analysis using an antisense cart-like RNA probe, we found that the cart-like transcript was predominantly expressed in both the Rohon-Beard neurons and trigeminal ganglia, suggesting the involvement of the cart-like gene in zebrafish neural development. |
| Databáze: | OpenAIRE |
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