Gut Digestive Function and Microbiome after Correction of Experimental Dysbiosis in Rats by Indigenous Bifidobacteria
| Autor: | Gruzdkov Aa, Yulia Dmitrieva, Anastasiya L Sepp, Nadezhda S Lavrenova, A N Suvorov, L. V. Gromova, Alena Karaseva, A. S. Alekseeva, Tatyana A Kramskaya, E I Ermolenko, Mariya P Kotyleva |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology intestinal digestive enzymes microbiome Gut flora Microbiology Article 03 medical and health sciences Virology Prevotella medicine Microbiome autoprobiotics lcsh:QH301-705.5 Bifidobacterium biology biology.organism_classification medicine.disease 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) Duodenum Alkaline phosphatase Maltase Dysbiosis |
| Zdroj: | Microorganisms, Vol 9, Iss 522, p 522 (2021) Microorganisms Volume 9 Issue 3 |
| ISSN: | 2076-2607 |
| Popis: | In recent years, great interest has arisen in the use of autoprobiotics (indigenous bacteria isolated from the organism and introduced into the same organism after growing). This study aimed to evaluate the effects of indigenous bifidobacteria on intestinal microbiota and digestive enzymes in a rat model of antibiotic-associated dysbiosis. Our results showed that indigenous bifidobacteria (the Bf group) accelerate the disappearance of dyspeptic symptoms in rats and prevent an increase in chyme mass in the upper intestine compared to the group without autoprobiotics (the C1 group), but significantly increase the mass of chyme in the colon compared to the C1 group and the control group (healthy animals). In the Bf group in the gut microbiota, the content of opportunistic bacteria (Proteus spp., enteropathogenic Escherichia coli) decreased, and the content of some beneficial bacteria (Bifidobacterium spp., Dorea spp., Blautia spp., the genus Ruminococcus, Prevotella, Oscillospira) changed compared to the control group. Unlike the C1 group, in the Bf group there was no decrease in the specific activities of maltase and alkaline phosphatase in the mucosa of the upper intestine, but the specific activity of maltase was decreased in the colon chyme compared to the control and C1 groups. In the Bf group, the specific activity of aminopeptidase N was reduced in the duodenum mucosa and the colon chyme compared to the control group. We concluded that indigenous bifidobacteria can protect the microbiota and intestinal digestive enzymes in the intestine from disorders caused by dysbiosis however, there may be impaired motor function of the colon. |
| Databáze: | OpenAIRE |
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