Production and Characterization of Fusion Proteins Containing Transferrin and Nerve Growth Factor
| Autor: | P. Lynch, S. D. Putney, E. Park, J. P. McGrath, A. J. Schutz, J. George, R. M. Starzyk, T. Lee |
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| Rok vydání: | 1998 |
| Předmět: |
chemistry.chemical_classification
Drug Carriers Recombinant Fusion Proteins Transferrin Brain Pharmaceutical Science Enzyme-Linked Immunosorbent Assay Transferrin receptor Biological activity Transfection Biology Polymerase Chain Reaction Fusion protein Drug Delivery Systems Nerve growth factor nervous system chemistry Biochemistry Blood-Brain Barrier Neurotrophic factors Receptors Transferrin Gene expression Humans Nerve Growth Factors |
| Zdroj: | Journal of Drug Targeting. 6:53-64 |
| ISSN: | 1029-2330 1061-186X |
| Popis: | To explore the ability to use genetic fusions of transferrin as a carrier for brain targeting and delivery, a series of fusion proteins containing both human nerve growth factor (NGF) and human transferrin was produced in mammalian cells. A protein in which the hinge region from human IgG3 joined the carboxyl terminus of NGF and the amino terminus of transferrin formed a covalent homodimer, bound human transferrin receptor, and retained full NGF in PC 12 cells. In contrast, proteins in which polypeptide dimerization was not induced or in which NGF was fused through its amino terminus had greatly reduced NGF activity. The ability to maintain both biologically active NGF and transferrin as part of a fusion protein may offer a novel way to deliver NGF and other neurotrophic factors to the central nervous system. |
| Databáze: | OpenAIRE |
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