Deoxyribonucleic acid methyl transferases 3a and 3b associate with the nuclear orphan receptor COUP-TFI during gene activation
| Autor: | Rozenn Gallais, Florence Demay, Peter Barath, Laurence Finot, Renata Jurkowska, Rémy Le Guével, Frédérique Gay, Albert Jeltsch, Raphaël Métivier, Gilles Salbert |
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| Přispěvatelé: | Interactions cellulaires et moléculaires (ICM), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Transcriptional Activation
DNMT3A Gene [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Biology MESH: COUP Transcription Factor I DNA Methyltransferase 3A 03 medical and health sciences Mice Endocrinology Epigenetics of physical exercise Chlorocebus aethiops Animals MESH: Animals DNA (Cytosine-5-)-Methyltransferases Vitronectin Promoter Regions Genetic Molecular Biology MESH: Mice 030304 developmental biology Regulation of gene expression Orphan receptor 0303 health sciences COUP Transcription Factor I 030302 biochemistry & molecular biology [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology General Medicine Methylation Molecular biology MESH: Cercopithecus aethiops MESH: Gene Expression Regulation MESH: Vitronectin MESH: DNA (Cytosine-5-)-Methyltransferase MESH: COS Cells MESH: Promoter Regions (Genetics) DNA demethylation Gene Expression Regulation DNA methylation COS Cells embryonic structures Thymine-DNA glycosylase |
| Zdroj: | Molecular Endocrinology-Baltimore Molecular Endocrinology-Baltimore-, Endocrine Society, 2007, 21 (9), pp.2085-98. ⟨10.1210/me.2006-0490⟩ Molecular Endocrinology-Baltimore-, 2007, 21 (9), pp.2085-98. ⟨10.1210/me.2006-0490⟩ |
| ISSN: | 0888-8809 |
| DOI: | 10.1210/me.2006-0490⟩ |
| Popis: | International audience; Transcriptional activation of silent genes can require the erasure of epigenetic marks such as DNA methylation at CpGs (cytosine-guanine dinucleotide). Active demethylation events have been observed, and associated processes are repeatedly suspected to involve DNA glycosylases such as mCpG binding domain protein 4, thymine DNA glycosylase (TDG), Demeter, and repressor of silencing 1. A complete characterization of the molecular mechanisms occurring in metazoan is nonetheless awaited. Here, we report that activation of the endogenous vitronectin gene in P19 cells by the nuclear receptor chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI) is observed in parallel with the recruitment of TDG and p68 RNA helicase, two components of a putative demethylation complex. Interestingly, when activated, the vitronectin gene was loaded with DNA methyltransferases 3a and 3b (Dnmt3a/b), and a strand-biased decrease in CpG methylation was detected. Dnmt3a was further found to associate with COUP-TFI and TDG in vivo, and cotransfection experiments demonstrated that Dnmt3a/b can enhance COUP-TFI-mediated activation of a methylated reporter gene. These results suggest that Dnmt3a/b could cooperate with the orphan receptor COUP-TFI to regulate transcription of the vitronectin gene. |
| Databáze: | OpenAIRE |
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