Global mRNA and miRNA Analysis Reveal Key Processes in the Initial Response to Infection with WSSV in the Pacific Whiteleg Shrimp
| Autor: | Eduarda M. Santos, Kelly S. Bateman, Karen Moore, Grant D. Stentiford, Charles R. Tyler, Audrey Farbos, Diana Minardi, Stuart Ross, Rebecca S. Millard, Lisa K. Bickley, Ronny van Aerle |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
invertebrate White spot syndrome RNA-Seq Models Biological Microbiology Article Animal Diseases Transcriptome 03 medical and health sciences DSCAM White spot syndrome virus 1 Penaeidae Virology microRNA Whiteleg shrimp Animals Gene silencing RNA Messenger 030102 biochemistry & molecular biology biology Gene Expression Profiling Computational Biology High-Throughput Nucleotide Sequencing Viral Load biology.organism_classification QR1-502 Shrimp Cell biology MicroRNAs 030104 developmental biology Infectious Diseases aquaculture Host-Pathogen Interactions RNA-seq transcriptome |
| Zdroj: | Viruses, Vol 13, Iss 1140, p 1140 (2021) Viruses Volume 13 Issue 6 |
| ISSN: | 1999-4915 |
| Popis: | White Spot Disease (WSD) presents a major barrier to penaeid shrimp production. Mechanisms underlying White Spot Syndrome Virus (WSSV) susceptibility in penaeids are poorly understood due to limited information related to early infection. We investigated mRNA and miRNA transcription in Penaeus vannamei over 36 h following infection. Over this time course, 6192 transcripts and 27 miRNAs were differentially expressed—with limited differential expression from 3–12 h post injection (hpi) and a more significant transcriptional response associated with the onset of disease symptoms (24 hpi). During early infection, regulated processes included cytoskeletal remodelling and alterations in phagocytic activity that may assist WSSV entry and translocation, novel miRNA-induced metabolic shifts, and the downregulation of ATP-dependent proton transporter subunits that may impair cellular recycling. During later infection, uncoupling of the electron transport chain may drive cellular dysfunction and lead to high mortalities in infected penaeids. We propose that post-transcriptional silencing of the immune priming gene Dscam (downregulated following infections) by a novel shrimp miRNA (Pva-pmiR-78 upregulated) as a potential mechanism preventing future recognition of WSSV that may be suppressed in surviving shrimp. Our findings improve our understanding of WSD pathogenesis in P. vannamei and provide potential avenues for future development of prophylactics and treatments. |
| Databáze: | OpenAIRE |
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