Mesenchymal stromal cell characteristics vary depending on their origin
| Autor: | Kornelius Wiechmann, Jürgen Funk, Mathias Leddin, Carsten Stein, Christoffer von Schwerin, Ralf Huss, Heike Wegmeyer, Saskia Knothe, Karin Kuentzer, Julia Hupfeld, Jennifer Kuhlen, Anna Katharina Nisslbeck, Markus Neubauer, Ann-Marie Bröske |
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| Rok vydání: | 2013 |
| Předmět: |
Stromal cell
Cellular differentiation Placenta Karyotype Cell- and Tissue-Based Therapy Gene Expression Bone Marrow Cells Biology Regenerative medicine Umbilical Cord Original Research Reports Pregnancy medicine Humans Regeneration Cell Lineage Amnion Progenitor cell Cells Cultured Cell Proliferation Regeneration (biology) Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Cell Biology Hematology Cell biology medicine.anatomical_structure Immunology Cytokines Intercellular Signaling Peptides and Proteins Female Bone marrow Developmental Biology |
| Zdroj: | Stem cells and development. 22(19) |
| ISSN: | 1557-8534 |
| Popis: | Mesenchymal stromal cells (MSCs) are rare progenitor cells that can be isolated from various tissues. They exhibit multilineage differentiation potential, support regenerative processes, and interact with various immune cells. Therefore, MSCs represent a promising tool for regenerative medicine. However, source-dependent and donor-dependent differences of MSC properties, including implications on their clinical application are still largely unknown. We evaluated MSCs derived from perinatal tissues umbilical cord (UC) and amniotic membrane (AM) in comparison to adult MSCs from bone marrow (BM), which were used as gold standard. We found genetic background-independent differences between MSCs from UC and AM. While AM- and UC-MSCs were closer to each other than to BM-MSCs, they also exhibited differences between each other. AM-MSCs from different donors but not UC-MSCs displayed high interdonor variability. In addition, we show that although all MSCs expressed similar surface markers, MSC populations from UC and AM showed differential profiles of gene expression and paracrine factor secretion to BM-derived MSCs. Notably, pathway analysis of gene expression data revealed intriguing differences between MSCs suggesting that MSCs from UC and AM possess in general a higher potential of immunomodulatory capacity, whereas BM-MSCs showed a higher potential of supporting regenerative processes as exemplified by neuronal differentiation and development. These differences between perinatal and BM-derived MSCs may be relevant for clinical applications. |
| Databáze: | OpenAIRE |
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