Biocompatibility and degradation of poly(ether-ester) microspheres: in vitro and in vivo evaluation
| Autor: | J.M. Bezemer, R. van Dijkhuizen-Radersma, P.E Kaim, S. C. Hesseling, K. de Groot |
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| Rok vydání: | 2002 |
| Předmět: |
Materials science
Time Factors Biocompatibility Polyesters Biophysics Phthalic Acids Bioengineering Ether Biocompatible Materials In Vitro Techniques Polyethylene Glycols Biomaterials chemistry.chemical_compound Hydrolysis In vivo Polymer chemistry Copolymer Animals Cytotoxicity Polyethylene Terephthalates In vitro Microspheres Biodegradation Environmental chemistry Mechanics of Materials Gamma Rays Ceramics and Composites Microscopy Electron Scanning Rabbits Ethylene glycol Nuclear chemistry Ethers |
| Zdroj: | Biomaterials. 23(24) |
| ISSN: | 0142-9612 |
| Popis: | Microspheres of a hydrophobic and a hydrophilic poly(ether-ester) copolymer were evaluated for their in vitro and in vivo biocompatibility and degradation. The microspheres prior to and after sterilization were tested for in vitro cytotoxicity. The in vivo biocompatibility of the poly(ethylene glycol) terephthalate and poly(butylene terephthalate) (PEGT/PBT) microspheres was evaluated subcutaneously and intramuscularly for 24 weeks in rabbits. The in vivo degradation of the microspheres was studied microscopically and compared to the in vitro degradation. The in vitro and in vivo studies showed the biocompatibility of the microspheres of both the hydrophobic and the hydrophilic PEGT/PBT copolymer. Extracts of these microspheres showed no cytotoxic reactivity in the in vitro cytotoxicity test. Sterilization of the microspheres by gamma irradiation did not affect the cytotoxicity. PEGT/PBT microspheres injected subcutaneously and intramuscularly in rabbits showed a mild tissue response in vivo, in terms of the inflammatory response, the foreign body reaction and the granulation tissue response. Although an in vitro degradation experiment showed a decrease in molecular weight due to hydrolysis, the in vivo degradation of the microspheres was slower than previously published. |
| Databáze: | OpenAIRE |
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