The concept of in vivo airway tissue engineering
Autor: | Wolfgang Harringer, Silvia Baiguera, Tetsuhiko Go, Kaj Fried, Paolo Macchiarini, Philipp Jungebluth, Susanne Möller, Kristín Rós Kjartansdóttir, Ana I. Teixeira, Heike Nave, Massimo Osvaldo Jaus, Vanessa Lundin, Augustinus Bader, Mei Ling Lim |
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Rok vydání: | 2012 |
Předmět: |
Male
Sus scrofa Biophysics Bioengineering Cell Separation Biology Biomaterials Tissue engineering In vivo Animals Humans RNA Messenger Progenitor cell Cells Cultured Inflammation Decellularization Tissue Engineering Regeneration (biology) Epithelial Cells Mesenchymal Stem Cells Flow Cytometry Biomechanical Phenomena Cell biology Trachea Transplantation Gene Expression Regulation Mechanics of Materials Ceramics and Composites Respiratory epithelium Female Stem cell Biomedical engineering |
Zdroj: | Biomaterials. 33:4319-4326 |
ISSN: | 0142-9612 |
Popis: | We investigated whether decellularized pig tracheas could regenerate in vivo, without being recellularized before transplantation, using the own body as bioreactor. Decellularized pig tracheal scaffolds were intraoperative conditioned with mononuclear cells and growth and differentiation factors. During the postoperative period, the in situ regeneration was boosted by administering bioactive molecules to promote peripheral mobilization and differentiation of stem/progenitor cells and ultimately the regenerative process. Results revealed, after 2 weeks, a nearly normal trachea, with respiratory epithelium and a double-banded cartilage but without any mechanical differences compared to the native tissue. The growth factor administration resulted in a mobilization of progenitor and stem cells into the peripheral circulation and in an up-regulation of anti-apoptotic genes. Isolated stem/progenitor cells could be differentiated in vitro into several cell types, proving their multipotency. We provide evidence that the own body can be used as bioreactor to promote in vivo tissue engineering replacement. Moreover, we demonstrated the beneficial effect of additional pharmaceutical intervention for an improved engraftment of the transplant. |
Databáze: | OpenAIRE |
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