Variation in Large‐Conductance, Calcium‐Activated Potassium Channels from Hair Cells Along the Chicken Basilar Papilla
| Autor: | Paul A. Fuchs, R. K. Duncan |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
BK channel Patch-Clamp Techniques Large-Conductance Calcium-Activated Potassium Channel beta Subunits Physiology Models Biological Potassium Channels Calcium-Activated Internal medicine Hair Cells Auditory medicine Animals Homeostasis Large-Conductance Calcium-Activated Potassium Channels Cloning Molecular Large-Conductance Calcium-Activated Potassium Channel alpha Subunits Organ of Corti Electrical tuning biology Alternative splicing Genetic Variation Original Articles Calcium-activated potassium channel Potassium channel Electrophysiology Major duodenal papilla Kinetics Endocrinology medicine.anatomical_structure Animals Newborn biology.protein Biophysics Calcium Hair cell Tonotopy Chickens |
| Zdroj: | The Journal of Physiology. 547:357-371 |
| ISSN: | 1469-7793 0022-3751 |
| DOI: | 10.1113/jphysiol.2002.029785 |
| Popis: | The mechanism for electrical tuning in non-mammalian hair cells rests within the widely diverse kinetics of functionally distinct, large-conductance potassium channels (BK), thought to result from alternative splicing of the pore-forming alpha subunit and variable co-expression with an accessory beta subunit. Inside-out patches from hair cells along the chicken basilar papilla revealed 'tonotopic' gradations in calcium sensitivity and deactivation kinetics. The resonant frequency for the hair cell from which the patch was taken was estimated from deactivation rates, and this frequency reasonably matched that predicted from the originating cell's tonotopic location. The rates of deactivation for native BK channels were much faster than rates reported for cloned chicken BK channels including both alpha and beta subunits. This result was surprising since patches were pulled from hair cells in the apical half of the papilla where beta subunits are most highly expressed. Heterogeneity in the properties of native chicken BK channels implies a high degree of molecular variation and hinders our ability to identify those molecular constituents. |
| Databáze: | OpenAIRE |
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