Altered expression of the kisspeptin/KISS1R and neurokinin B/NK3R systems in mural granulosa and cumulus cells of patients with polycystic ovarian syndrome

Autor: Ainhoa Fernández-Atucha, Nicolás Prados, Francisco M. Pinto, Manuel Fernández-Sánchez, Víctor Blasco, Luz Candenas, Manuel Tena-Sempere
Přispěvatelé: Ministerio de Economía y Competitividad (España)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Granulosa cells
Kisspeptin
endocrine system diseases
Neurokinin B
Stimulation
Pathogenesis
chemistry.chemical_compound
0302 clinical medicine
Receptor
Genetics (clinical)
Cells
Cultured
media_common
Kisspeptins
030219 obstetrics & reproductive medicine
Cumulus Cells
Obstetrics and Gynecology
Receptors
Neurokinin-3
General Medicine
female genital diseases and pregnancy complications
Reproductive Physiology and Disease
medicine.anatomical_structure
Female
Polycystic Ovary Syndrome
Adult
endocrine system
medicine.medical_specialty
Polycystic ovarian syndrome
Cumulus cells
media_common.quotation_subject
03 medical and health sciences
Follicle
Young Adult
Internal medicine
Genetics
medicine
Humans
Ovulation
business.industry
Oocyte
030104 developmental biology
Endocrinology
Cross-Sectional Studies
Reproductive Medicine
chemistry
Gene Expression Regulation
Case-Control Studies
business
Developmental Biology
Receptors
Kisspeptin-1
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
Popis: PURPOSE: The neurokinin B (NKB)/NK3 receptor (NK3R) and kisspeptin (KISS1)/kisspeptin receptor (KISS1R), two systems essential for reproduction, are present in human granulosa cells (GCs) of healthy women and contribute to the control of fertility, at least partially, by acting on the gonads. However, little is known about the expression of these systems in GCs of women with polycystic ovarian syndrome (PCOS). The aim of this study was to analyze the expression of NKB/NK3R and KISS1/KISS1R in mural granulosa (MGCs) and cumulus cells (CCs) of PCOS women. METHODS: A cross-sectional study was performed in 46 healthy women and 43 PCOS women undergoing controlled ovarian stimulation. MGCs and CCs were collected from pre-ovulatory follicles after transvaginal ultrasound-guided oocyte retrieval and the expression of the genes encoding NKB (TAC3), NK3R (TACR3), KISS1, and its receptor (KISS1R) was analyzed using real-time quantitative RT-PCR. RESULTS: TAC3, TACR3, and KISS1 mRNA levels were decreased in MGCs and CCs of PCOS women. TAC3 positively correlated with KISS1 in MGCs of healthy women and TACR3 was positively associated with KISS1R in CCs from healthy women. These associations were not observed in PCOS women. CONCLUSION: The NKB/NK3R and KISS1/KISS1R systems are dysregulated in MGCs and CCs of PCOS women. The lower expression of these systems in GCs could contribute to the abnormal follicle development and defective ovulation that characterize the pathogenesis of PCOS.
Databáze: OpenAIRE
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