The effects of intra-rectal and intra-peritoneal application of Origanum onites L. essential oil on 2,4,6-trinitrobenzenesulfonic acid-induced colitis in the rat

Autor: Serap Işiksoy, K. Hüsnü Can Başer, Emine Dündar, Esra Gurlek Olgun, Mine Kurkcuoglu, Cengiz Bal
Přispěvatelé: Anadolu Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, Kürkçüoğlu, Mine, Başer, K. Hüsnü Can
Rok vydání: 2006
Předmět:
Pathology
Antioxidant
medicine.medical_treatment
Pharmacology
Toxicology
Antioxidants
Dexamethasone
law.invention
Rats
Sprague-Dawley
chemistry.chemical_compound
law
Origanum
Treatment of inflammatory bowel disease
Intestinal Mucosa
biology
Origanum onites
General Medicine
6-Trinitrobenzenesulfonic acid-induced colitis
Colitis
Intercellular Adhesion Molecule-1
Immunohistochemistry
Myeloperoxidase
Injections
Intraperitoneal
medicine.drug
medicine.medical_specialty
Colon
Gas Chromatography-Mass Spectrometry
Pathology and Forensic Medicine
2
4
6-Trinitrobenzenesulfonic acid
Administration
Rectal
medicine
Oils
Volatile
Animals
Essential oil
Peroxidase
Ethanol
business.industry
Cell Biology
medicine.disease
biology.organism_classification
Mucus
Rats
Disease Models
Animal
chemistry
Trinitrobenzenesulfonic Acid
biology.protein
Colitis
Ulcerative
business
2
4
6-Trinitrobenzenesulfonic Acid-Induced Colitis
Zdroj: Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie. 59(6)
ISSN: 0940-2993
0002-5550
Popis: WOS: 000255508800008
PubMed ID: 18222658
The aim of the present study is to investigate the treatment efficiency of intra-rectal (IR) and intra-peritoneal (W) application of Origanum onites essential oil (OOEO), which is a well-known antioxidant, in the colitis model induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and ethanol (E) in comparison with dexamethasone therapy through the morphologic damage score. Monoclonal antibodies against intercellular adhesion molecule-1 (ICAM-1, CD54), antirat granulocytes, and myeloperoxidase (MPO), were also investigated immunohistochemically. There was a significant difference in terms of ulceration, mucus cell depletion, inflammatory cell infiltration, vascular dilatation (P < 0.001), crypt abscesses (p < 0.01), and edema (p < 0.05) between OOEO-1 mg/kg-IR and control colitis groups. A significant difference was encountered in terms of mucus cell depletion, crypt abscesses, inflammatory cell infiltration, vascular dilatation (p < 0.01), and ulceration (p < 0.05) between the OOEO-0.1 mg/kg-IR and control colitis groups. A significant difference was noticed in terms of ulceration, inflammatory cell infiltration, mucus cell depletion (P < 0.001), vascular dilatation (p < 0.01), and mucosal atrophy (p < 0.05) between the OOEO-1 mg/kg-IP and control colitis groups. There was a significant difference in terms of ulceration, mucus cell depletion, inflammatory cell infiltration (p < 0.001), crypt abscesses, vascular dilatation (p < 0.01), and mucosal atrophy (p < 0.05) between the OOEO-0.1 mg/kg-IP and control colitis groups. No significant difference was determined in terms of ulceration, inflammatory cyst, mucosal atrophy, edema, and vascular dilatation between the dexamethazone and control colitis groups (p > 0.05). Under the present conditions, we concluded that IR and IP OOEO treatment, applied at the dosage of 0.1 or 1 mg/kg/day, have a significant protective effect on the colonic injury
Databáze: OpenAIRE
Externí odkaz: