Delivery Mode Affects Intestinal Microbial Composition and the Development of Intestinal Epithelial Cells
| Autor: | Xue Han, Da-you Cheng, Linzheng Lyu, Yuanyuan Li, Li Liu, Meng Zhang, Shilong Jiang, Xiaohong Zhou, Haiyue Niu, Jiliang Zhang, Lanwei Zhang, Jiancun Pan, Pimin Gong, Shiwei Chen |
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| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
intestinal microbiota Bifidobacterium bifidum biology Cell growth Vaginal delivery ved/biology ved/biology.organism_classification_rank.species Membrane transport delivery modes biology.organism_classification Delivery mode Microbiology bacterial screening QR1-502 infant intestinal epithelial cells cell proliferation In vivo Cell culture Bacteria Original Research |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 12 (2021) |
| ISSN: | 1664-302X |
| Popis: | BackgroundThe infant’s intestine contains diverse microbiota, which play an important role in an infant’s health.ObjectiveThis study aimed to analyze the different intestinal microbiota and their function in two delivery modes [vaginal delivery and cesarean section (C-section)] and to investigate the proprieties of bacteria associated with vaginal delivery on the development of intestinal epithelial cells in rat pups.Materials and MethodsWe evaluated the intestinal microbial diversity of the stool samples of 51 infants of subjects who underwent vaginal delivery and C-section by sequencing the V4 regions of the 16S rRNA gene and predicted the function of the microbiotas. The infant stool microbiota in the vaginal delivery group was associated with the digestive system and cell growth and death, whereas that of the C-section group was associated with membrane transport. Then, we isolated the strains based on function prediction.ResultsA total of 95 strains were isolated in the vaginal delivery group. Bifidobacterium bifidum FL-228.1 (FL-228.1) was screened and selected owing to its good surface hydrophobicity, bacterial survivability in the simulated gastrointestinal condition and adhesion ability to the IEC-6 cell line as well as owing to the development of intestinal epithelial cells. Furthermore, in vivo experiments revealed that FL-228.1 exhibited favorable effects on the development of intestinal epithelial cells in rat pups.ConclusionThe results of this study indicate an apparent difference in the bacterial composition of the stool samples collected from infants of the two delivery modes. By analyzing and screening the bacteria in infant stool samples, we found that one strain, i.e., B bifidum FL-228.1, exhibited favorable effects on the development of intestinal epithelial cells. |
| Databáze: | OpenAIRE |
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