Bcl-2 Gene Therapy Prevents Aminoglycoside-Induced Degeneration of Auditory and Vestibular Hair Cells
| Autor: | Peter K. Plinkert, Hinrich Staecker, Susanna C. Pfannenstiel, Mark Praetorius, Douglas E. Brough |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty Cell Survival Physiology Myosins Adenoviridae Hair Cells Vestibular Mice Speech and Hearing Ototoxicity In vivo Evoked Potentials Auditory Brain Stem In Situ Nick-End Labeling otorhinolaryngologic diseases Animals Medicine Hearing Disorders Vestibular Hair Cell Antibacterial agent business.industry Aminoglycoside Auditory Threshold Genetic Therapy medicine.disease Sensory Systems Anti-Bacterial Agents Mice Inbred C57BL Hair Cells Auditory Outer Auditory brainstem response medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Otorhinolaryngology Myosin VIIa Female Gentamicin sense organs Hair cell Gentamicins business medicine.drug |
| Zdroj: | Audiology and Neurotology. 14:254-266 |
| ISSN: | 1421-9700 1420-3030 |
| DOI: | 10.1159/000192953 |
| Popis: | To evaluate the protective effects of bcl-2, we have developed an in vivo model of gentamicin ototoxicity in C57BL/6 mice using intratympanic delivery of gentamicin. Hair cell survival was evaluated using myosin VIIa immunohistochemistry, cytocochleogram and auditory brainstem response (ABR) testing. At 10 days after gentamicin application, a consistent loss of outer hair cells was seen. Mice were pretreated with an adenovector expressing human bcl-2 (Ad.11D.bcl-2) or a control vector (Ad.11D). Seventy-two hours after vector delivery mice were treated with intratympanic gentamicin and evaluated at 10 days after ototoxin delivery. Pretreatment with Ad.11D.bcl-2 resulted in morphologic protection of hair cells and preservation of hearing thresholds measured by ABR. |
| Databáze: | OpenAIRE |
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