Region-specific changes in activities of cell death-related proteases and nitric oxide metabolism in rat brain in a chronic unpredictable stress model
| Autor: | Natalia Pasikova, Mikhail V. Onufriev, I. P. Levshina, Yulia Moiseeva, Alexey Rukhlenko, Alexey Piskunov, A. O. Tishkina, Mikhail Stepanichev, Natalia V. Gulyaeva |
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| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Proteases Hippocampus Cell Count Nitric Oxide Biochemistry Cathepsin B Open field Cellular and Molecular Neuroscience Internal medicine medicine Animals Chronic stress Rats Wistar Cell damage Nitrites Brain Chemistry Neocortex Nitrates biology Cell Death Calpain Caspase 3 Body Weight Organ Size medicine.disease Immunohistochemistry Rats Endocrinology medicine.anatomical_structure biology.protein Neurology (clinical) Nitric Oxide Synthase Corticosterone Stress Psychological Peptide Hydrolases |
| Zdroj: | Metabolic brain disease. 27(4) |
| ISSN: | 1573-7365 |
| Popis: | Effects of a chronic combined unpredictable stress on activities of two cell death-related proteases, calpain and cathepsin B, were studied along with indices of nitrergic system in rat brain structures. Male Wistar rats were subjected to a 2-week-long combined stress (combination of unpaired flash light and moderate footshock associated with a white noise session). Stress resulted in a significant loss in the body and thymus weight and increased defecation in the open field test, though neither motor and exploratory activity, nor plasma corticosterone differed from the respective control levels. Decreased calpain activity and increased cathepsin B activity were demonstrated in the hippocampus of stressed rats (previously we have shown that caspase-3 activity was significantly suppressed in the brain of rats subjected to same type of stress). A significant reduction in the number of NOS-containing neurons was accompanied by a chronic stressinduced decline in NOS activity in the neocortex. Similar changes were observed in the hippocampus. However, levels of NO metabolites were elevated in both structures. Thus, stress-induced structural modifications in the brain may be mediated by disturbances in the nitrergic system and increased lysosomal proteolysis. |
| Databáze: | OpenAIRE |
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