Identification of the Cellular Receptor of Clostridium spiroforme Toxin
| Autor: | Thilo Nölke, Claudia Wilczek, Panagiotis Papatheodorou, Daniel Hornuss, Klaus Aktories, Gregor Guttenberg, Carsten Schwan |
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| Rok vydání: | 2012 |
| Předmět: |
Botulinum Toxins
Clostridium spiroforme Bacterial Toxins Immunology Clostridium difficile toxin A Enterotoxin medicine.disease_cause Microbiology Cell Line Enterotoxins Clostridium medicine Humans ADP Ribose Transferases Host cell surface Cellular Microbiology: Pathogen-Host Cell Molecular Interactions biology Clostridioides difficile Toxin Clostridium difficile biology.organism_classification Recombinant Proteins Infectious Diseases Receptors LDL Bacillus megaterium Clostridium Infections Clostridium botulinum Parasitology Caco-2 Cells human activities HeLa Cells Protein Binding |
| Zdroj: | Infection and Immunity. 80:1418-1423 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.06378-11 |
| Popis: | Clostridium spiroforme produces the binary actin-ADP-ribosylating toxin CST ( C. spiroforme toxin), which has been proposed to be responsible for diarrhea, enterocolitis, and eventually death, especially in rabbits. Here we report on the recombinant production of the enzyme component (CSTa) and the binding component (CSTb) of C. spiroforme toxin in Bacillus megaterium . By using the recombinant toxin components, we show that CST enters target cells via the lipolysis-stimulated lipoprotein receptor (LSR), which has been recently identified as the host cell receptor of the binary toxins Clostridium difficile transferase (CDT) and Clostridium perfringens iota toxin. Microscopic studies revealed that CST, but not the related Clostridium botulinum C2 toxin, colocalized with LSR during toxin uptake and traffic to endosomal compartments. Our findings indicate that CST shares LSR with C. difficile CDT and C. perfringens iota toxin as a host cell surface receptor. |
| Databáze: | OpenAIRE |
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