Rhodamine bound maghemite as a long-term dual imaging nanoprobe of adipose tissue-derived mesenchymal stromal cells
Autor: | Josef Skopalik, Ivan Justan, Vratislav Cmiel, Katerina Polakova, Jan Solar, Markéta Havrdová, Ivo Provaznik, Zenon Starčuk, Massimiliano Magro, David Milde |
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Rok vydání: | 2016 |
Předmět: |
Male
Fluorophore Cell Survival Mesenchymal stromal cells Intracellular Space Biophysics Analytical chemistry Nanoprobe 02 engineering and technology 010402 general chemistry 01 natural sciences Fluorescence law.invention Rhodamine Iron oxide nanoparticles chemistry.chemical_compound Confocal microscopy law Dual contrast agents Intracellular fluorescent labels Stem cell tracking Adipose Tissue Dextrans Female Humans Magnetite Nanoparticles Mesenchymal Stem Cells Molecular Imaging Molecular Probes Rhodamines Spectrometry Fluorescence Spectrometry Chemistry Mesenchymal stem cell General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences Molecular imaging 0210 nano-technology Intracellular |
Zdroj: | ResearcherID |
ISSN: | 1432-1017 0175-7571 |
Popis: | In the last few years, magnetically labeled cells have been intensively explored, and non-invasive cell tracking and magnetic manipulation methods have been tested in preclinical studies focused on cell transplantation. For clinical applications, it is desirable to know the intracellular pathway of nanoparticles, which can predict their biocompatibility with cells and the long-term imaging properties of labeled cells. Here, we quantified labeling efficiency, localization, and fluorescence properties of Rhodamine derivatized superparamagnetic maghemite nanoparticles (SAMN-R) in mesenchymal stromal cells (MSC). We investigated the stability of SAMN-R in the intracellular space during a long culture (20 days). Analyses were based on advanced confocal microscopy accompanied by atomic absorption spectroscopy (AAS) and magnetic resonance imaging. SAMN-R displayed excellent cellular uptake (24 h of labeling), and no toxicity of SAMN-R labeling was found. 83% of SAMN-R nanoparticles were localized in lysosomes, only 4.8% were found in mitochondria, and no particles were localized in the nucleus. On the basis of the MSC fluorescence measurement every 6 days, we also quantified the continual decrease of SAMN-R fluorescence in the average single MSC during 18 days. An additional set of analyses showed that the intracellular SAMN-R signal decrease was minimally caused by fluorophore degradation or nanoparticles extraction from the cells, main reason is a cell division. The fluorescence of SAMN-R nanoparticles within the cells was detectable minimally for 20 days. These observations indicate that SAMN-R nanoparticles have a potential for application in transplantation medicine. |
Databáze: | OpenAIRE |
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