Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319
| Autor: | Zhao Dong Meng, Shou Ping Lu, Wen Cai Li, Chun Hua Mu, Yu Yang, Xia Liu, Fa Jun Zhang, Guang Cun Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:Biotechnology Biology Plant disease resistance lcsh:Plant culture 01 natural sciences Genome law.invention Crop 03 medical and health sciences Inbred strain law lcsh:TP248.13-248.65 Botany lcsh:SB1-1110 Gene Polymerase chain reaction General Environmental Science Genetics Bacterial artificial chromosome resistance genes food and beverages inbred line Maize 030104 developmental biology General Earth and Planetary Sciences Primer (molecular biology) bacterial artificial chromosome library 010606 plant biology & botany |
| Zdroj: | Crop Breeding and Applied Biotechnology, Vol 16, Iss 1, Pp 22-27 (2016) Crop Breeding and Applied Biotechnology, Volume: 16, Issue: 1, Pages: 22-27, Published: MAR 2016 Crop Breeding and Applied Biotechnology v.16 n.1 2016 Crop Breeding and Applied Biotechnology Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB |
| ISSN: | 1984-7033 1518-7853 |
| Popis: | Zea mays L. has been the most cultivated crop and the crop with the largest yield in China since 2012. We constructed a bacterial artificial chromosome (BAC) library for the maize inbred line Qi319, which may be used as a key source for disease-resistant maize breeding in China. The BAC contains 270,720 clones, with an average insert size of 90 kb. The coverage of the library is about 10.43 genome equivalents when considering a haploid genome size of 2300 Mb, providing a 99.99% likelihood of isolating any maize gene or sequence in the library. An average of 12 clones were obtained by polymerase chain reaction screening by using primer pairs linked to the genes for resistance to maize southern rust and rough dwarf. The results indicate that the library can satisfy the requirements for recovering specific sequences. The library is available to researchers to whom it may be of interest. |
| Databáze: | OpenAIRE |
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