Chorionic Gonadotropin-β Modulates Epithelial-Mesenchymal Transition in Colorectal Carcinoma Metastasis
| Autor: | Mishie Tanino, Akinobu Taketomi, Hideki Kawamura, Hiroshi Nishihara, Shinya Tanaka, Yuji Konishi, Futoshi Kawamata, Tadashi Yoshida, Toshiya Kamiyama, Shinji Kohsaka, Lei Wang, Cheng Liu, Yasutaka Kato, Shigenori Homma, Masumi Tsuda |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Epithelial-Mesenchymal Transition Colorectal cancer medicine.drug_class Biology Pathology and Forensic Medicine Metastasis Human chorionic gonadotropin Mice 03 medical and health sciences 0302 clinical medicine Cell Movement Transforming Growth Factor beta Cell Line Tumor medicine Animals Humans Chorionic Gonadotropin beta Subunit Human Neoplasm Invasiveness Clinical significance Epithelial–mesenchymal transition Phosphorylation Cell Proliferation Prognosis medicine.disease 030104 developmental biology Cell culture 030220 oncology & carcinogenesis embryonic structures Disease Progression Cancer research Gonadotropin Colorectal Neoplasms Signal Transduction Transforming growth factor |
| Zdroj: | The American Journal of Pathology. 188:204-215 |
| ISSN: | 0002-9440 |
| DOI: | 10.1016/j.ajpath.2017.08.034 |
| Popis: | Ectopic production of free β human chorionic gonadotropin (hCGβ) has been associated with aggressive behavior in non-trophoblastic tumors. hCGβ shares common evolutionary sequences with transforming growth factor-β (TGF-β), which represents a major driving force of epithelial-to-mesenchymal transition (EMT). In this study, we examined the biological roles of hCGβ during EMT and its clinical significance in colorectal cancer (CRC) progression. Eighty CRC specimens and 54 preoperative serum samples were analyzed. hCGβ-overexpressing human CRC cell lines were examined for invasiveness and tumorigenicity, and the expression of EMT-associated genes was investigated. In human CRC, histologic hCGβ positivity [13/80 (16.3%)] was lower than serologic hCGβ positivity [13/54 (24.1%)]. However, it was significantly correlated with several clinicopathological features and unfavorable outcome (P 0.05). hCGβ-overexpressing cell lines had increased invasiveness, migratory ability, and metastatic potential in mice (P 0.01). Western blot, PCR, and microarray analyses showed hCGβ altered expression of EMT-related genes, including E-cadherin, phosphorylated SMAD2, SNAIL, and TWIST. hCGβ-induced SNAIL and TWIST overexpression levels were reversible by type I and type II TGF-β receptor inhibitors (P 0.05). hCGβ thus induces EMT via the TGF-β signaling pathway, and it may represent a molecular target in CRC treatment. |
| Databáze: | OpenAIRE |
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