Adipose Gene Expression Profile Changes With Lung Allograft Reperfusion

Autor: G. Singh, Jason D. Christie, Matthew Bacchetta, Selim M. Arcasoy, Y. Suzuki, Carly A. D’Errico, Christian A. Bermudez, Anthony W. Ferrante, Jon T. Giles, Joshua M. Diamond, Amika McBurnie, Edward Cantu, Melanie Rushefski, Frank D'Ovidio, Michelle Oyster, Laurel Kalman, David J. Lederer, J. McDonnough, Matthew Lippel, Joshua R. Sonett
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Pathology
medicine.medical_treatment
Adipose tissue
Pilot Projects
0302 clinical medicine
Risk Factors
Gene expression
Immunology and Allergy
Medicine
Pharmacology (medical)
Prospective Studies
Membrane Glycoproteins
biology
respiratory system
Allografts
Prognosis
Serum Amyloid P-Component
C-Reactive Protein
Adipose Tissue
Female
Signal transduction
Lung Transplantation
Adult
medicine.medical_specialty
Primary Graft Dysfunction
Lung injury
Article
03 medical and health sciences
Humans
Lung transplantation
Obesity
Gene
Serum amyloid P component
Aged
Transplantation
business.industry
Membrane Proteins
030104 developmental biology
030228 respiratory system
Case-Control Studies
Reperfusion
Cancer research
biology.protein
Transcriptome
business
Biomarkers
Follow-Up Studies
Zdroj: American Journal of Transplantation. 17:239-245
ISSN: 1600-6135
Popis: Obesity is a risk factor for primary graft dysfunction (PGD), a form of lung injury resulting from ischemia-reperfusion after lung transplantation, but the impact of ischemia-reperfusion on adipose tissue is unknown. We evaluated differential gene expression in thoracic visceral adipose tissue (VAT) before and after lung reperfusion. Total RNA was isolated from thoracic VAT sampled from six subjects enrolled in the Lung Transplant Body Composition study before and after allograft reperfusion and quantified using the Human Gene 2.0 ST array. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed enrichment for genes involved in complement and coagulation cascades and Jak-STAT signaling pathways. Overall, 72 genes were upregulated and 56 genes were downregulated in the postreperfusion time compared with baseline. Long pentraxin-3, a gene and plasma protein previously associated with PGD, was the most upregulated gene (19.5-fold increase, p = 0.04). Fibronectin leucine-rich transmembrane protein-3, a gene associated with cell adhesion and receptor signaling, was the most downregulated gene (4.3-fold decrease, p = 0.04). Ischemia-reperfusion has a demonstrable impact on gene expression in visceral adipose tissue in our pilot study of nonobese, non-PGD lung transplant recipients. Future evaluation will focus on differential adipose tissue gene expression and the development of PGD after transplant.
Databáze: OpenAIRE
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