Characterization of Gemcitabine Loaded Polyhydroxybutyrate Coated Magnetic Nanoparticles for Targeted Drug Delivery
| Autor: | Maryam Parsian, Ufuk Gündüz, Pelin Mutlu, Serap Yalcin |
|---|---|
| Přispěvatelé: | Tıp Fakültesi, Serap Yalçın / 0000-0002-9584-266X |
| Rok vydání: | 2020 |
| Předmět: |
Antimetabolites
Antineoplastic Cancer Research Polyesters Hydroxybutyrates Nanoparticle 02 engineering and technology 010402 general chemistry Deoxycytidine 01 natural sciences Structure-Activity Relationship Breast cancer Drug Delivery Systems Prohibitins Tumor Cells Cultured medicine Humans Particle Size Magnetite Nanoparticles Cell Proliferation Pharmacology Tumor microenvironment Dose-Response Relationship Drug Molecular Structure Cell growth Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Gemcitabine In vitro 0104 chemical sciences MCF-7 Targeted drug delivery Magnetic nanoparticles Cancer research Molecular Medicine Drug Screening Assays Antitumor 0210 nano-technology medicine.drug |
| Zdroj: | Anti-Cancer Agents in Medicinal Chemistry. 20:1233-1240 |
| ISSN: | 1871-5206 |
| DOI: | 10.2174/1871520620666200310091026 |
| Popis: | Background: Targeted drug delivery is one of the recent hot topics in cancer therapy. Because of having a targeting potential under the magnetic field and a suitable surface for the attachment of different therapeutic moieties, magnetic nanoparticles are widely studied for their applications in medicine. Objective: Gemcitabine loaded polyhydroxybutyrate coated magnetic nanoparticles (Gem-PHB-MNPs) were synthesized and characterized for the treatment of breast cancer by the targeted drug delivery method. Methods: The characterization of nanoparticles was confirmed by FTIR, XPS, TEM, and spectrophotometric analyses. The cytotoxicities of drug-free nanoparticles and Gemcitabine loaded nanoparticles were determined with cell proliferation assay using SKBR-3 and MCF-7 breast cancer cell lines. Result: The release of Gemcitabine from PHB-MNPs indicated a pH-dependent pattern, which is a desirable release characteristic, since the pH of the tumor microenvironment and endosomal structures are acidic, while bloodstream and healthy-tissues are neutral. Drug-free PHB-MNPs were not cytotoxic to the SKBR-3 and MCF- 7 cells, whereas the Gemcitabine loaded PHB-MNPs was about two-fold as cytotoxic with respect to free Gemcitabine. In vitro targeting ability of PHB-MNPs was shown under the magnetic field. Conclusion: Considering these facts, we may suggest that these nanoparticles can be a promising candidate for the development of a novel targeted drug delivery system for breast cancer. |
| Databáze: | OpenAIRE |
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