Molecular Mechanism of the Syk Activation Switch
| Autor: | Emily Tsang, Shaan Gandhi, David E. Shaw, Marie Dinh, J. Michael Bradshaw, Eva Papp, Joyce K. Y. Tse, Anthony M. Giannetti, Sandra Wang, Hoangdung Ho |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Amino Acid Motifs
Syk chemical and pharmacologic phenomena Models Biological environment and public health Biochemistry Substrate Specificity Substrate-level phosphorylation Gene expression Humans Syk Kinase Phosphorylation Tyrosine Receptor Molecular Biology B-Lymphocytes ZAP-70 Protein-Tyrosine Kinase Chemistry Kinase Autophosphorylation Intracellular Signaling Peptides and Proteins hemic and immune systems Cell Biology Protein-Tyrosine Kinases Kinetics enzymes and coenzymes (carbohydrates) Spectrometry Fluorescence Mutagenesis Immune System biological phenomena cell phenomena and immunity Peptides Gene Deletion |
| Zdroj: | Journal of Biological Chemistry. 283:32650-32659 |
| ISSN: | 0021-9258 |
| Popis: | Many immune signaling pathways require activation of the Syk tyrosine kinase to link ligation of surface receptors to changes in gene expression. Despite the central role of Syk in these pathways, the Syk activation process remains poorly understood. In this work we quantitatively characterized the molecular mechanism of Syk activation in vitro using a real time fluorescence kinase assay, mutagenesis, and other biochemical techniques. We found that dephosphorylated full-length Syk demonstrates a low initial rate of substrate phosphorylation that increases during the kinase reaction due to autophosphorylation. The initial rate of Syk activity was strongly increased by either pre-autophosphorylation or binding of phosphorylated immune tyrosine activation motif peptides, and each of these factors independently fully activated Syk. Deletion mutagenesis was used to identify regions of Syk important for regulation, and residues 340-356 of the SH2 kinase linker region were identified to be important for suppression of activity before activation. Comparison of the activation processes of Syk and Zap-70 revealed that Syk is more readily activated by autophosphorylation than Zap-70, although both kinases are rapidly activated by Src family kinases. We also studied Syk activity in B cell lysates and found endogenous Syk is also activated by phosphorylation and immune tyrosine activation motif binding. Together these experiments show that Syk functions as an "OR-gate" type of molecular switch. This mechanism of switch-like activation helps explain how Syk is both rapidly activated after receptor binding but also sustains activity over time to facilitate longer term changes in gene expression. |
| Databáze: | OpenAIRE |
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